School of Nutrition, Chung Shan Medical University and Department of Nutrition, Chung Shan Medical University Hospital, No. 110, Section 1, Jianguo North Road, Taichung 40201, Taiwan.
Food Funct. 2012 Jan;3(1):49-57. doi: 10.1039/c1fo10209e. Epub 2011 Nov 18.
The aim of this work was to study the effects of garcinol and pterostilbene on cell proliferation and adipogenesis in 3T3-L1 cells. The results showed that garcinol and pterostilbene decreased the cell population growth and caused cell cycle arrest at the G2/M phase in 3T3-L1 preadipocytes. During adipocyte differentiation, both garcinol and pterostilbene had inhibitory effects on fat droplet formation and triacylglycerol accumulation. The data indicated that garcinol and pterostilbene could inhibit the glycerol-3-phosphate dehydrogenase (GPDH) activity by 97.8 and 61.5%, respectively, as compared to the control. Both garcinol and pterostilbene significantly attenuated the protein expressions of PPARγ and C/EBPα during 3T3-L1 adipocyte differentiation. Moreover, garcinol and pterostilbene caused an inhibition of lipid accumulation in the 3T3-L1 adipocyte differentiation phase. Garcinol and pterostilbene also significantly up-regulated the gene expression of adiponectin as well as down-regulated the gene expressions of leptin, resistin, and fatty acid synthase (FAS) in 3T3-L1 adipocyte differentiation. In 3T3-L1 adipocytes, garcinol significantly down-regulated the protein expressions of PPARγ and FAS as well as up-regulated the protein expressions of adipose triglyceride lipase (ATGL) and adiponectin. Garcinol also significantly up-regulated the gene expression of adiponectin as well as down-regulated the gene expressions of leptin and FAS. These results suggest that garcinol and pterostilbene have anti-adipogenic effects on preadipocytes and adipocytes.
本研究旨在探讨 Garcinol 和 Pterostilbene 对 3T3-L1 前脂肪细胞增殖和脂肪生成的影响。结果表明，Garcinol 和 Pterostilbene 可抑制 3T3-L1 前脂肪细胞的群体生长，并导致细胞周期停滞在 G2/M 期。在脂肪细胞分化过程中，Garcinol 和 Pterostilbene 均可抑制脂肪滴形成和三酰基甘油积累。数据表明，与对照组相比，Garcinol 和 Pterostilbene 可分别抑制甘油-3-磷酸脱氢酶（GPDH）活性 97.8%和 61.5%。Garcinol 和 Pterostilbene 在 3T3-L1 脂肪细胞分化过程中均可显著下调 PPARγ 和 C/EBPα 的蛋白表达。此外，Garcinol 和 Pterostilbene 可抑制 3T3-L1 脂肪细胞分化过程中的脂质积累。Garcinol 和 Pterostilbene 还可显著上调脂肪细胞因子基因表达，下调瘦素、抵抗素和脂肪酸合成酶（FAS）基因表达。在 3T3-L1 脂肪细胞中，Garcinol 可显著下调 PPARγ 和 FAS 的蛋白表达，上调脂肪甘油三酯脂肪酶（ATGL）和脂联素的蛋白表达。Garcinol 还可显著上调脂联素基因表达，下调瘦素和 FAS 基因表达。这些结果表明，Garcinol 和 Pterostilbene 对前脂肪细胞和脂肪细胞具有抗脂肪生成作用。
