Gülnar Birgül, Canatar İpek, Özdaş Sibel
Department of Bioengineering, Faculty of Engineering Sciences, Adana Alpaslan Türkeş Science and Technology University, Adana, Turkey.
Turk J Biol. 2023 Feb 21;47(2):141-157. doi: 10.55730/1300-0152.2649. eCollection 2023.
Since obesity causes at least 2.8 million death each year and is a major risk factor for many diseases, it is critical to evaluate alternative treatment approaches. In this context, studies on the research of natural product-based therapeutics in the fight against obesity are increasing. In this study, it was aimed to evaluate the antiadipogenic and antiobesogenic efficacy of pterostilbene a natural phenolic compound in 3T3-L1 cells. The mature 3T3-L1 adipocytes were exposed to pterostilbene at different concentrations and half-maximum inhibitory concentrations (IC) were determined by MTT assay. Oil-Red-O staining was applied to determine lipid accumulation. Phase contrast microscopy, Giemsa, F-actin and DAPI staining were applied to examine the efficacy of pterostilbene on the morphology of 3T3-L1 adipocyte cells. Moreover, expressions of and in relation to insulin resistance were evaluated using immunofluorescent staining and qRT-PCR. Pterostilbene caused no significant cytotoxicity towards preadipocytes at concentrations ≤7.5 -M and the percentage of viable cells remained above about 86% for 24 h, 48 h and 72 h (p > 0.05). Therefore, pterostilbene treatment at 5 and 7.5 -M was used in the subsequent experiments as safe dosages. In addition, it was observed that pterostilbene treatment reduced lipid accumulation in adipocyte differentiation. Adipocytes treated with a dose of 7.5 -M for 14 days showed less intense lipid deposition and a more spindle-like morphology compared to the adipocytes treated with a dose of 5 -M. Especially on the 14th day, actin filaments were filamentous in adipocytes treated with pterostilbene 7.5 -M compared to the adipocytes treated with a dose of 5 -M; the filaments were similarly oriented as in preadipocytes, and chromatin condensation was observed to be quite high. Our data suggests that the pterostilbene supplementation may help weight control and the antiadipogenic and that antiobesogenic activity is mediated in part by reduction of lipid accumulation and induction of and levels.
由于肥胖每年导致至少280万人死亡，且是许多疾病的主要风险因素，因此评估替代治疗方法至关重要。在此背景下，关于基于天然产物的治疗方法对抗肥胖的研究不断增加。在本研究中，旨在评估天然酚类化合物紫檀芪在3T3-L1细胞中的抗脂肪生成和抗肥胖功效。将成熟的3T3-L1脂肪细胞暴露于不同浓度的紫檀芪，并通过MTT法测定半数最大抑制浓度（IC）。应用油红O染色来确定脂质积累。应用相差显微镜、吉姆萨染色、F-肌动蛋白和DAPI染色来检查紫檀芪对3T3-L1脂肪细胞形态的功效。此外，使用免疫荧光染色和qRT-PCR评估与胰岛素抵抗相关的 和 的表达。紫檀芪在浓度≤7.5 μM时对前脂肪细胞无明显细胞毒性，活细胞百分比在24小时、48小时和72小时内保持在约86%以上（p>0.05）。因此，在后续实验中使用5 μM和7.5 μM的紫檀芪处理作为安全剂量。此外，观察到紫檀芪处理减少了脂肪细胞分化中的脂质积累。与用5 μM剂量处理的脂肪细胞相比，用7.5 μM剂量处理14天的脂肪细胞显示出较弱的脂质沉积和更呈纺锤状的形态。特别是在第14天，与用5 μM剂量处理的脂肪细胞相比，用7.5 μM紫檀芪处理的脂肪细胞中的肌动蛋白丝呈丝状；这些丝的方向与前脂肪细胞中的相似，并且观察到染色质凝聚相当高。我们的数据表明，补充紫檀芪可能有助于控制体重，其抗脂肪生成和抗肥胖活性部分是通过减少脂质积累以及诱导 和 水平来介导的。
