Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
Small. 2012 Jan 23;8(2):214-9. doi: 10.1002/smll.201101925. Epub 2011 Nov 17.
A facile silica coating significantly enhances the thermal stability and polymerase chain reaction (PCR) compatibility of oligonucleotide-gold nanoparticle conjugates, thus enabling colorimetric detection of PCR results in a closed-tube format. This method is specific, sensitive, and generally applicable. Its simplicity, visual readout, and carryover contamination-free features hold promise for point-of-care or on-site DNA testing.
一种简便的硅涂层显著提高了寡核苷酸-金纳米粒子缀合物的热稳定性和聚合酶链反应（PCR）兼容性，从而能够在封闭管形式下进行 PCR 结果的比色检测。该方法具有特异性、灵敏度和通用性。其简单、直观的读取和无交叉污染的特点有望用于即时或现场 DNA 检测。
