基于实时 PCR 样灵敏度的单核苷酸多态性的比色检测。
Colorimetric detection of single-nucleotide polymorphisms with a real-time PCR-like sensitivity.
机构信息
Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
出版信息
Chem Commun (Camb). 2012 Oct 21;48(82):10225-7. doi: 10.1039/c2cc35070j.
PMID:22968418
Abstract
Coupling gold nanoparticle-based ligation to a ligation chain reaction enables colorimetric detection of single-nucleotide polymorphisms with a real-time PCR-like sensitivity. One mutant in 2000 copies of a wild-type gene can be detected in 20-100 pg of PCR-amplified genomic DNA samples.
摘要
将基于金纳米粒子的连接与连接链反应偶联，实现了对单核苷酸多态性的比实时 PCR 更灵敏的比色检测。在 20-100 pg 的 PCR 扩增基因组 DNA 样本中，可以检测到 2000 个野生型基因中的一个突变体。
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