Wang Xicheng, Kayesh Emrul, Han Jian, Liu Chonghuai, Wang Chen, Song Changnian, Ge Anjing, Fang Jinggui
College of Horticulture, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu, People's Republic of China,
Mol Biol Rep. 2014 Jul;41(7):4397-412. doi: 10.1007/s11033-014-3311-6. Epub 2014 Apr 13.
Microarray analysis of genes can provide individual gene-expression profiles and new insights for elucidating biological mechanisms responsible for fruit development. To obtain an overall view on expression profiles of metabolism-related genes involved in fruit development of table and wine grapes, a microarray system comprising 15,403 ESTs was used to compare the expressed genes. The expression patterns from the microarray analysis were validated with quantitative real-time polymerase chain reaction analysis of 18 selected genes of interest. During the entire fruit development stage, 2,493 genes exhibited at least 2.0-fold differences in expression levels with 1,244 genes being up-regulated and 1,249 being down-regulated. Following gene ontology analysis, only 929 differentially expressed (including 403 up-regulated and 526 down-regulated) genes were annotated in table and wine grapes. These differentially expressed genes were found to be mainly involved in carbohydrate metabolism, biosynthesis of secondary metabolites as well as energy, lipid and amino acid metabolism via KEGG. Our results provide new insights into the molecular mechanisms and expression profiles of genes in the fruit development stage of table and wine grapes.
基因微阵列分析能够提供个体基因表达谱，并为阐明果实发育背后的生物学机制带来新的见解。为全面了解鲜食葡萄和酿酒葡萄果实发育过程中与代谢相关基因的表达谱，我们使用了一个包含15403个EST的微阵列系统来比较表达的基因。微阵列分析得到的表达模式通过对18个选定的目标基因进行定量实时聚合酶链反应分析进行了验证。在整个果实发育阶段，2493个基因的表达水平表现出至少2.0倍的差异，其中1244个基因上调，1249个基因下调。经过基因本体分析，在鲜食葡萄和酿酒葡萄中仅注释了929个差异表达基因（包括403个上调基因和526个下调基因）。通过KEGG分析发现，这些差异表达基因主要参与碳水化合物代谢、次生代谢物生物合成以及能量、脂质和氨基酸代谢。我们的研究结果为鲜食葡萄和酿酒葡萄果实发育阶段的基因分子机制和表达谱提供了新的见解。
