Mor I, Cheung E C, Vousden K H
The Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1BD, UK.
Cold Spring Harb Symp Quant Biol. 2011;76:211-6. doi: 10.1101/sqb.2011.76.010868. Epub 2011 Nov 17.
Regulation of glucose metabolism is a crucial aspect of cell physiology in normal and disease conditions. Many regulatory events are involved in determining the metabolic fate of glucose and the pathways into which it is directed. The first reaction that commits glucose to the glycolytic pathway is catalyzed by the enzyme phosphofructokinase-1 (PFK-1) and is tightly regulated. One of the most potent activators of PFK-1 is fructose 2,6 bisphosphate (F2,6BP) and its cellular levels are correlated with glycolytic flux. F2,6BP is synthesized and degraded by a family of bifunctional enzymes-the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (PFKFB). The interplay among F2,6BP levels, the enzymes that generate and degrade it, and PFK-1 activity has important consequences for several different aspects of cell metabolism as well as for systemic metabolic conditions. TIGAR, a recently identified F2,6 bisphosphatase (F2,6BPase), could also contribute to this complexity and participate in shaping the metabolic profile of the cell.
在正常和疾病状态下，葡萄糖代谢的调节是细胞生理学的一个关键方面。许多调节事件参与决定葡萄糖的代谢命运及其所进入的途径。使葡萄糖进入糖酵解途径的第一个反应由磷酸果糖激酶-1（PFK-1）催化，并且受到严格调控。PFK-1最有效的激活剂之一是果糖2,6-二磷酸（F2,6BP），其细胞水平与糖酵解通量相关。F2,6BP由一类双功能酶——6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶（PFKFB）合成和降解。F2,6BP水平、生成和降解它的酶以及PFK-1活性之间的相互作用，对细胞代谢的几个不同方面以及全身代谢状况都有重要影响。TIGAR是一种最近发现的果糖2,6-二磷酸酶（F2,6BPase），它也可能导致这种复杂性，并参与塑造细胞的代谢特征。
