School of Environmental Science, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia.
Water Sci Technol. 2011;64(3):587-94. doi: 10.2166/wst.2011.565.
Nitrogen removal performance and the ammonia-oxidising bacterial (AOB) community were assessed in the batch loaded 1.3 ha saturated surface vertical flow wetland at CSBP Ltd, a fertiliser and chemical manufacturer located in Kwinana, Western Australia. From September 2008 to October 2009 water quality was monitored and sediment samples collected for bacterial analyses. During the period of study the wetland received an average inflow of 1,109 m3/day with NH3-N = 40 mg/L and NO3-N = 23 mg/L. Effluent NH3-N and NO3-N were on average 31 and 25 mg/L, respectively. The overall NH3-N removal rate for the period was 1.2 g/m2/day indicating the nitrifying capacity of the wetland. The structure of the AOB community was analysed using group specific primers for the ammonia monooxygenase gene (amoA) by terminal restriction fragment length polymorphism and by clone libraries to identify key members. The majority of sequences obtained were most similar to Nitrosomonas sp. while Nitrosospira sp. was less frequent. Another two vertical flow wetlands, 0.8 ha each, were commissioned at CSBP in July 2009, since then the wetland in this study has received nitrified effluent from these two new cells.
在位于西澳大利亚州奎那纳的化肥和化学制品制造商 CSBP 有限公司，我们评估了 1.3 公顷饱和表面垂直流湿地在批量加载时的脱氮性能和氨氧化细菌 (AOB) 群落。从 2008 年 9 月到 2009 年 10 月，监测了水质并收集了沉积物样本进行细菌分析。在研究期间，湿地平均每天接收 1109 立方米的流量，NH3-N = 40 毫克/升，NO3-N = 23 毫克/升。出水 NH3-N 和 NO3-N 的平均值分别为 31 和 25 毫克/升。该时期的整体 NH3-N 去除率为 1.2 克/平方米/天，表明湿地具有硝化能力。使用氨单加氧酶基因 (amoA) 的特异性引物通过末端限制性片段长度多态性和克隆文库分析了 AOB 群落的结构，以鉴定关键成员。获得的大多数序列与硝化单胞菌最相似，而硝化螺菌则较少。2009 年 7 月，CSBP 又建成了另外两个 0.8 公顷的垂直流湿地，从那时起，本研究中的湿地就接收了这两个新单元的硝化出水。
