Ma Ying, Bu Lingyi, Hua Xiaosu
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2011 Oct;28(5):997-1000.
Leishmaniasis (Kala-azar) from different endemic regions of China expresses different clinic and epidemiological features, and traditionally is classified as hilly, plain and desert types/foci. We concentrated our review on whether the pathogens from those foci were different at molecular level, if so, whether there are were molecular markers readily identifiable by molecular technologies. This was a review of a 20-year search for such markers by using kinetoplastic DNA (kDNA), nDNA hybridization, PCR-SSCP, RAPD and sequence analysis of SSU rDNA variable regions and LACK gene. The results showed that heterogeneities at molecular level exist in Leishmania isolated from different foci of China, which could be used as markers for different types of Leishmaniasis in China.
来自中国不同流行地区的利什曼病（黑热病）表现出不同的临床和流行病学特征，传统上分为丘陵型、平原型和荒漠型/疫源地。我们的综述聚焦于这些疫源地的病原体在分子水平上是否存在差异，如果存在差异，分子技术是否能轻易识别出分子标记。这是一篇对利用动质体DNA（kDNA）、核DNA杂交、PCR-SSCP、RAPD以及小亚基核糖体DNA（SSU rDNA）可变区和LACK基因序列分析来寻找此类标记的20年研究的综述。结果表明，从中国不同疫源地分离出的利什曼原虫在分子水平上存在异质性，这可作为中国不同类型利什曼病的标记。
