Hu X, Lu H, Luo P, Wang Z, Hu X, Yi T
Laboratory of Parasitology, West China University of Medical Sciences, Chengdu.
Chin Med Sci J. 1992 Jun;7(2):63-6.
kDNA sequence homology of Leishmania donovani isolates from three types of kala-azar foci in China were analyzed by using dot and Southern hybridization with biotin- and 32P-labelled probes. The results revealed kDNA sequence heterogeneity among Leishmania donovani isolates from the three kala-azar foci: sequence homology between isolates of hill and desert foci was higher than that between hill and plain foci isolates. The kDNA hybridization technique was also found to be specific and sensitive for direct identification of Leishmania in animal tissues. In a preliminary survey, kDNA hybridization of cutaneous tissue blots of 71 dogs from endemic regions showed a positive rate of 40.8%, and the rate of double positive cases (touch blot hybridization and bone marrow smear) reached 91.3%. The direct identification of Leishmania in tissues by kDNA hybridization seems to be a useful and convenient method for epidemiological study and clinical diagnosis, especially for species/strain characterization.
利用生物素和32P标记的探针，通过斑点杂交和Southern杂交分析了来自中国三种黑热病疫源地的杜氏利什曼原虫分离株的kDNA序列同源性。结果显示，来自三种黑热病疫源地的杜氏利什曼原虫分离株之间存在kDNA序列异质性：山区和沙漠疫源地分离株之间的序列同源性高于山区和平原疫源地分离株之间的同源性。还发现kDNA杂交技术对于直接鉴定动物组织中的利什曼原虫具有特异性和敏感性。在一项初步调查中，对来自流行地区的71只犬的皮肤组织印迹进行kDNA杂交，阳性率为40.8%，双阳性病例（触片杂交和骨髓涂片）率达到91.3%。通过kDNA杂交直接鉴定组织中的利什曼原虫似乎是一种用于流行病学研究和临床诊断的有用且便捷的方法，特别是对于物种/菌株鉴定。
