[气相色谱-氢火焰离子化检测器法研究醒脑静注射液中冰片在大鼠体内的药代动力学]

[Study on pharmacokinetics of borneol in rats injected with novel-xingnaojing by GC-FID].

作者信息

Lu Yang, Du Shouying, Chen Xiaolan, Li Pengyue, Zhai Yongsong, Wu Qing, Li Dongxue

机构信息

Beijing University of Chinese Medicine, Beijing 100102, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2011 Aug;36(16):2200-2.

PMID:22097329

Abstract

OBJECTIVE

To develop a GC-FID method for the determination of borneol concentration in rat plasma and to investigate the pharmacokinetics after injection of novel-Xingnaojing.

METHOD

Novel-Xingnaojing was injected via by caudal vein injection. The blood samples were collected by posterior orbital venous plexus approach at 0.5, 1, 3, 5, 8, 12, 20, 30, 45 min. The drug in plasma was extracted with ethyl acetate and then detected by GC-FID, octadecane was used as the internal standard. The pharmacokinetic parameters were calculated by the software of Kinetica.

RESULT

The calibration curve was good linear in the range of 1.67-16.67 mg x L(-1). The extraction recoveries of low, medium and high concentration were (92.81 +/- 1.11)%, (85.38 +/- 0.86)% and (84.58 +/- 0.58)%, respectivley. And the RSDs of within-day and between-day were below 3.00%. Plasma concentration of borneol was consistent with the two-compartment open model. The pharmacokinetic parameters were that the t1/2alpha was (1.18 +/- 0.20) min, the t1/2beta was (22.27 +/- 6.85) min, the C(max)(Calc) was (18.76 +/- 2.10) mg x L(-1), the MRT was (23.84 +/- 7.67) min(-1), and the AUC was (100.00 +/- 15.85) mg x min x L(-1).

CONCLUSION

The GC-FID method developed can be applied to determination and pharmacokinetics. The borneol in novel-Xingnaojing is distributed and metabolized fast after being administrated.

摘要

目的

建立气相色谱-氢火焰离子化检测器（GC-FID）法测定大鼠血浆中冰片浓度，并研究注射新型醒脑静后的药代动力学。

方法

通过尾静脉注射给予新型醒脑静。于0.5、1、3、5、8、12、20、30、45分钟经眶后静脉丛采血。血浆中的药物用乙酸乙酯萃取，然后用GC-FID检测，以十八烷为内标。药代动力学参数用Kinetica软件计算。

结果

校准曲线在1.67 - 16.67 mg·L⁻¹范围内线性良好。低、中、高浓度的萃取回收率分别为（92.81 ± 1.11）%、（85.38 ± 0.86）%和（84.58 ± 0.58）%。日内和日间相对标准偏差均低于3.00%。冰片血浆浓度符合二室开放模型。药代动力学参数为：t1/2α为（1.18 ± 0.20）分钟，t1/2β为（22.27 ± 6.85）分钟，C(max)(Calc)为（18.76 ± 2.10）mg·L⁻¹，MRT为（23.84 ± 7.67）分钟⁻¹，AUC为（100.00 ± 15.85）mg·min·L⁻¹。