Gao Huimin, Wu Xiyan, Li Zongyun, You Yun, Zhang Yi, Wang Zhimin
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2011 Aug;36(16):2207-10.
Eight compounds were isolated from Bufonis periostracum by repeated column chromatography on silica gel, ODS and Sephadex LH-20 and their structures were characterized as palmitatic acid cholesteryl ester (1), cholesterol (2), 5alpha, 8alpha-epidioxycholesta-6-en-3beta-ol (3), cholest-5-en-3beta, 7beta-diol (4), cholest-7-en-3beta, 5alpha, 6beta-triol (5), 3-octaddecyloxy-1, 2-propanediol (6), isisamide (7) and bufothionine (8) on the base of spectral analysis. Compounds 1-8 were isolated from Bufonis periostracum for the first time and compounds 3, 5, 6, 7 were obtained from Bufo bufo gargarizans and Bufo genus for the first time. The bioassays showed all tested samples displayed no antitumor activity against the cell lines such as A549, BeL 7402, HGC-27 and HL-60, except the control compound bufalin.
通过硅胶柱色谱、ODS柱色谱和葡聚糖凝胶LH - 20柱色谱反复分离，从蟾酥皮中分离得到8种化合物。基于光谱分析，它们的结构被鉴定为棕榈酸胆固醇酯（1）、胆固醇（2）、5α, 8α - 环氧胆甾 - 6 - 烯 - 3β - 醇（3）、胆甾 - 5 - 烯 - 3β, 7β - 二醇（4）、胆甾 - 7 - 烯 - 3β, 5α, 6β - 三醇（5）、3 - 十八烷氧基 - 1, 2 - 丙二醇（6）、异酰胺（7）和蟾硫因（8）。化合物1 - 8首次从蟾酥皮中分离得到，化合物3、5、6、7首次从中华大蟾蜍及蟾蜍属中获得。生物活性测定表明，除对照化合物蟾毒灵外，所有测试样品对A549、Bel 7402、HGC - 27和HL - 60等细胞系均无抗肿瘤活性。
