Singha Santiswarup, Dasgupta Anjan K, Datta Himadri
Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India.
J Nanosci Nanotechnol. 2011 Sep;11(9):7744-52. doi: 10.1166/jnn.2011.4729.
Citrate capped gold nanoparticles (GNP) are effective in masking protein amines. The extent of such masking is quantified using Fourier Transform Infra Red (FTIR) spectroscopy. A strong correlation is shown to exist between a shift of amide-II peak intensity (1600-1500 cm(-1)) caused by GNP and the number of exposed amines in a given protein. The result is validated using eight different proteins. The expected out-come of such masking is inhibition of interaction between any external ligand and such amines. The prediction is validated using a simple non-enzymatic glycation of clinically important protein like crystallin.
