Whiteley H E, Scott J R
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801.
Exp Eye Res. 1990 Oct;51(4):383-91. doi: 10.1016/0014-4835(90)90150-s.
Developing canine retina, fixed in Bouin's or Tellyesnizky's acetic alcoholic formalin, was examined with a battery of biotinylated lectins. ConA and WGA consistently bound to all retinal layers including the tapetum cellulosum, retinal pigment epithelium, inner and outer segments, plexiform layers and ganglion cells. Binding became more intense with increased age. S-WGA bound to apical portions of the retinal pigment epithelium and to outer segments. Outer segments also bound ConA, WGA and RCA. DBA preferentially labelled all inner segments, while PNA bound to a subpopulation of inner segments. RCA bound intensely to retinal vessels as did PNA after neuraminidase treatment. UEA did not consistently bind to any layers. There was transient binding by some lectins at different developmental stages. This study indicates that canine retina demonstrates differential expression of glycoproteins, as indicated by lectin binding, during development and that this expression is temporally and topographically regulated.
用一系列生物素化凝集素对固定于布安氏液或特利耶斯尼茨基氏醋酸酒精福尔马林的发育中的犬视网膜进行了检查。刀豆球蛋白A（ConA）和小麦胚凝集素（WGA）始终与包括纤维素绒毡层、视网膜色素上皮、内段和外段、神经纤维层和神经节细胞在内的所有视网膜层结合。随着年龄增长，结合变得更加明显。大豆凝集素（S-WGA）与视网膜色素上皮的顶端部分和外段结合。外段也与ConA、WGA和蓖麻凝集素（RCA）结合。双花扁豆凝集素（DBA）优先标记所有内段，而花生凝集素（PNA）与一部分内段结合。RCA与视网膜血管强烈结合，神经氨酸酶处理后的PNA也是如此。荆豆凝集素（UEA）并非始终与任何层结合。一些凝集素在不同发育阶段有短暂结合。这项研究表明，如凝集素结合所示，犬视网膜在发育过程中表现出糖蛋白的差异表达，并且这种表达在时间和空间上受到调控。
