Pang Ying, Li Min, Zhang Jian-Bo, Yao Bin
Department of Pediatrics, Sichuan Provincial People's Hospital, Chengdu, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2011 Nov;13(11):917-20.
To study the effects of early intervention on CD4+CD25+ regulatory T cells and TLR4 expression with Mycobacterium phlei F.U.36 in asthmatic mice.
Thirty female BALB/c mice were randomly divided into three groups: control, asthma model and Mycobacterium phlei F.U.36 treated asthma groups. Asthma was induced by sensitization and challenges with ovalbumin (OVA) in the later two groups. Mycobacterium phlei F.U.36 was intraperitoneally injected 2 weeks before the first sensitization (0.57 μg/time, once every other day for three times) in the intervention group. After 24 hrs of the last challenge, the mice were sacrificed and the left lung tissues were obtained for the observation of lung pathological changes. Splenic mononuclear cells were isolated. The percentage of CD4+CD25+ regulatory T cells in CD4+ T cells and the mean fluorescence intensity of TLR4 on CD4+ CD25+ T cells were detected by flow cytometry.
The percentage of CD4+CD25+ regulatory T cells in the asthma model group was significantly lower than that in the control group (P<0.01), but the mean fluorescence intensity of TLR4 on CD4+CD25+ regulatory T cells was not significantly different from the control group. The percentage of CD4+CD25+ regulatory T cells and the mean fluorescence intensity of TLR4 on CD4+CD25+ regulatory T cells increased significantly in asthmatic mice receiving Mycobacterium phlei F.U.36 treatment compared with the asthma group (P<0.01).
Early intervention with Mycobacterium phlei F.U.36 can increase TLR4 expression on CD4+CD25+ cells and the number of CD4+CD25+ regulatory T cells, and thus provides therapeutic effects in asthmatic mice.
研究草分枝杆菌F.U.36早期干预对哮喘小鼠CD4+CD25+调节性T细胞及Toll样受体4（TLR4）表达的影响。
将30只雌性BALB/c小鼠随机分为三组：对照组、哮喘模型组和草分枝杆菌F.U.36干预哮喘组。后两组通过卵清蛋白（OVA）致敏和激发诱导哮喘。干预组在首次致敏前2周腹腔注射草分枝杆菌F.U.36（0.57μg/次，隔日1次，共3次）。末次激发24小时后处死小鼠，取左肺组织观察肺组织病理变化。分离脾单个核细胞。采用流式细胞术检测CD4+T细胞中CD4+CD25+调节性T细胞的百分比以及CD4+CD25+T细胞上TLR4的平均荧光强度。
哮喘模型组CD4+CD25+调节性T细胞百分比显著低于对照组（P<0.01），但CD4+CD25+调节性T细胞上TLR4的平均荧光强度与对照组相比差异无统计学意义。与哮喘组相比，接受草分枝杆菌F.U.36治疗的哮喘小鼠CD4+CD25+调节性T细胞百分比及CD4+CD25+调节性T细胞上TLR4的平均荧光强度显著升高（P<0.01）。
草分枝杆菌F.U.36早期干预可增加CD4+CD25+细胞上TLR4表达及CD4+CD25+调节性T细胞数量，从而对哮喘小鼠产生治疗作用。
