Department of Biochemistry, Faculty of Science, Ege University, Bornova, Izmir 35100, Turkey.
Department of Chemistry, Faculty of Science, Ege University, Bornova, Izmir 35100, Turkey.
Biosensors (Basel). 2023 Mar 28;13(4):430. doi: 10.3390/bios13040430.
Herein, dendrimer-modified montmorillonite (Mt)-decorated poly-Ɛ-caprolactone (PCL) and chitosan (CHIT)-based nanofibers were prepared. Mt was modified with a poly(amidoamine) generation 1 (PAMAM) dendrimer, and the obtained PAMAM-Mt was incorporated into the PCL-CHIT nanofiber's structure. The PCL-CHIT/PAMAM-Mt nanofibers were conjugated with glutamate oxidase (GluO) to design a bio-based detection system for monosodium glutamate (MSG). PAMAM-Mt was added to the PCL-CHIT backbone to provide a multipoint binding side to immobilize GluO via covalent bonds. After the characterization of PCL-CHIT/PAMAM-Mt/GluO, it was calibrated for MSG. The linear ranges were determined from 0.025 to 0.25 mM MSG using PCL-CHIT/Mt/GluO and from 0.0025 to 0.175 mM MSG using PCL-CHIT/PAMAMG-Mt/GluO (with a detection limit of 7.019 µM for PCL-CHIT/Mt/GluO and 1.045 µM for PCL-CHIT/PAMAMG-Mt/GluO). Finally, PCL-CHIT/PAMAMG-Mt/GluO was applied to analyze MSG content in tomato soup without interfering with the sample matrix, giving a recovery percentage of 103.125%. Hence, the nanofiber modification with dendrimer-intercalated Mt and GluO conjugation onto the formed nanocomposite structures was performed, and the PCL-CHIT/PAMAM-Mt/GluO system was successfully developed for MSG detection.
本文制备了接枝树枝状大分子聚酰胺-胺（PAMAM）的蒙脱石（Mt）修饰的聚己内酯（PCL）和壳聚糖（CHIT）基纳米纤维。将 Mt 用聚（酰胺-胺）一代 1（PAMAM）树枝状大分子进行修饰，得到的 PAMAM-Mt 被掺入 PCL-CHIT 纳米纤维的结构中。将 PCL-CHIT/PAMAM-Mt 纳米纤维与谷氨酸氧化酶（GluO）缀合，设计了一种基于生物的检测系统用于检测谷氨酸钠（MSG）。PAMAM-Mt 被添加到 PCL-CHIT 主链中，通过共价键提供多点结合侧以固定 GluO。在对 PCL-CHIT/PAMAM-Mt/GluO 进行表征后，对其进行了 MSG 的校准。使用 PCL-CHIT/Mt/GluO 确定了从 0.025 到 0.25 mM MSG 的线性范围，使用 PCL-CHIT/PAMAMG-Mt/GluO 确定了从 0.0025 到 0.175 mM MSG 的线性范围（PCL-CHIT/Mt/GluO 的检测限为 7.019 µM，PCL-CHIT/PAMAMG-Mt/GluO 的检测限为 1.045 µM）。最后，将 PCL-CHIT/PAMAMG-Mt/GluO 应用于分析番茄汤中的 MSG 含量，而不会干扰样品基质，回收率为 103.125%。因此，对纳米纤维进行了修饰，用插层 Mt 的树枝状大分子对其进行了修饰，并将 GluO 接枝到形成的纳米复合材料结构上，成功开发了用于 MSG 检测的 PCL-CHIT/PAMAM-Mt/GluO 系统。
