基于有机-无机结构、电纺纳米纤维修饰电极上谷氨酸氧化酶的谷氨酸酶生物传感器的研制及其用于检测谷氨酸钠。

Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic-Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection.

机构信息

Department of Biochemistry, Faculty of Science, Ege University, Bornova, Izmir 35100, Turkey.

Department of Chemistry, Faculty of Science, Ege University, Bornova, Izmir 35100, Turkey.

出版信息

Biosensors (Basel). 2023 Mar 28;13(4):430. doi: 10.3390/bios13040430.

Herein, dendrimer-modified montmorillonite (Mt)-decorated poly-Ɛ-caprolactone (PCL) and chitosan (CHIT)-based nanofibers were prepared. Mt was modified with a poly(amidoamine) generation 1 (PAMAM) dendrimer, and the obtained PAMAM-Mt was incorporated into the PCL-CHIT nanofiber's structure. The PCL-CHIT/PAMAM-Mt nanofibers were conjugated with glutamate oxidase (GluO) to design a bio-based detection system for monosodium glutamate (MSG). PAMAM-Mt was added to the PCL-CHIT backbone to provide a multipoint binding side to immobilize GluO via covalent bonds. After the characterization of PCL-CHIT/PAMAM-Mt/GluO, it was calibrated for MSG. The linear ranges were determined from 0.025 to 0.25 mM MSG using PCL-CHIT/Mt/GluO and from 0.0025 to 0.175 mM MSG using PCL-CHIT/PAMAMG-Mt/GluO (with a detection limit of 7.019 µM for PCL-CHIT/Mt/GluO and 1.045 µM for PCL-CHIT/PAMAMG-Mt/GluO). Finally, PCL-CHIT/PAMAMG-Mt/GluO was applied to analyze MSG content in tomato soup without interfering with the sample matrix, giving a recovery percentage of 103.125%. Hence, the nanofiber modification with dendrimer-intercalated Mt and GluO conjugation onto the formed nanocomposite structures was performed, and the PCL-CHIT/PAMAM-Mt/GluO system was successfully developed for MSG detection.

本文制备了接枝树枝状大分子聚酰胺-胺（PAMAM）的蒙脱石（Mt）修饰的聚己内酯（PCL）和壳聚糖（CHIT）基纳米纤维。将 Mt 用聚（酰胺-胺）一代 1（PAMAM）树枝状大分子进行修饰，得到的 PAMAM-Mt 被掺入 PCL-CHIT 纳米纤维的结构中。将 PCL-CHIT/PAMAM-Mt 纳米纤维与谷氨酸氧化酶（GluO）缀合，设计了一种基于生物的检测系统用于检测谷氨酸钠（MSG）。PAMAM-Mt 被添加到 PCL-CHIT 主链中，通过共价键提供多点结合侧以固定 GluO。在对 PCL-CHIT/PAMAM-Mt/GluO 进行表征后，对其进行了 MSG 的校准。使用 PCL-CHIT/Mt/GluO 确定了从 0.025 到 0.25 mM MSG 的线性范围，使用 PCL-CHIT/PAMAMG-Mt/GluO 确定了从 0.0025 到 0.175 mM MSG 的线性范围（PCL-CHIT/Mt/GluO 的检测限为 7.019 µM，PCL-CHIT/PAMAMG-Mt/GluO 的检测限为 1.045 µM）。最后，将 PCL-CHIT/PAMAMG-Mt/GluO 应用于分析番茄汤中的 MSG 含量，而不会干扰样品基质，回收率为 103.125%。因此，对纳米纤维进行了修饰，用插层 Mt 的树枝状大分子对其进行了修饰，并将 GluO 接枝到形成的纳米复合材料结构上，成功开发了用于 MSG 检测的 PCL-CHIT/PAMAM-Mt/GluO 系统。