Department of Bioengineering, University of Pittsburgh, 3700 O'Hara Street, Pittsburgh, PA 15261, USA.
Acta Biomater. 2012 Feb;8(2):502-10. doi: 10.1016/j.actbio.2011.11.004. Epub 2011 Nov 11.
Most synthetic polymers lack biological and chemical functionalities. This lack of functionality restricts the polymer properties and prevents them from controlling specific cell-material interactions. Polymers with free functional groups allow facile modifications, which can be used to control the biointerface. Here we created a functionalizable polymer, poly(fumaroyl bioxirane) maleate (PFM), with three free functional groups--hydroxyl, carboxyl and alkenyl--for bone tissue engineering. PFM was readily synthesized in two steps. PFM showed strain-dependent moduli with mechanical strength approaching native bones. PFM supported the adhesion, spreading, proliferation, and maturity of rat calvarial osteoblasts. The alkaline phosphatase activity of osteoblasts on PFM was significantly higher than that on tissue-culture-treated polystyrene in vitro. The physical, mechanical, and biological properties of PFM can be modulated by various functionalizations to explore methods to improve bone tissue engineering and regenerative medicine in general.
大多数合成聚合物缺乏生物和化学功能。这种功能的缺乏限制了聚合物的性质，并阻止它们控制特定的细胞-材料相互作用。具有游离官能团的聚合物允许进行方便的修饰，可用于控制生物界面。在这里，我们创建了一种可功能化的聚合物，马来酸聚（富马酰基恶唑啉）（PFM），它具有三个游离官能团——羟基、羧基和烯基——用于骨组织工程。PFM 可以很容易地通过两步合成。PFM 表现出应变依赖性模量，机械强度接近天然骨骼。PFM 支持大鼠颅骨成骨细胞的黏附、铺展、增殖和成熟。成骨细胞在 PFM 上的碱性磷酸酶活性显著高于在体外组织培养处理的聚苯乙烯上的活性。PFM 的物理、机械和生物学性质可以通过各种功能化进行调节，以探索改善骨组织工程和再生医学的方法。
