Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Corps, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
J Proteomics. 2012 Feb 2;75(4):1099-118. doi: 10.1016/j.jprot.2011.10.023. Epub 2011 Nov 7.
Two-dimensional gel electrophoresis (2-DE) was used to analyze the proteins related to floral reversion in Dimocarpus longan Lour. Proteins were extracted from buds undergoing the normal process of flowering and from those undergoing floral reversion in three developing stages in D. longan. Differentially expressed proteins were identified from the gels after 2-DE analysis, which were confirmed using matrix-assisted laser desorption/ionization-time of flying-mass spectroscopy and protein database search. A total of 39 proteins, including 18 up-regulated and 21 down-regulated proteins, were classified into different categories, such as energy and substance metabolism, protein translation, secondary metabolism, phytohormone, cytoskeleton structure, regulation, and stress tolerance. Among these, the largest functional class was associated with primary metabolism. Down-regulated proteins were involved in photosynthesis, transcription, and translation, whereas up-regulated proteins were involved in respiration. Decreased flavonoid synthesis and up-regulated GA20ox might be involved in the floral reversion process. Up-regulated 14-3-3 proteins played a role in the regulation of floral reversion in D. longan by responding to abiotic stress. Observations via transmission electron microscopy revealed the ultrastructure changes in shedding buds undergoing floral reversion. Overall, the results provided insights into the molecular basis for the floral reversion mechanism in D. longan.
二维凝胶电泳（2-DE）用于分析龙眼（Dimocarpus longan Lour.）花逆转相关的蛋白质。从龙眼正常开花过程中的芽和正在经历花逆转的芽中提取蛋白质，共分为三个发育阶段。通过 2-DE 分析从凝胶中鉴定出差异表达的蛋白质，并用基质辅助激光解吸/电离-飞行时间质谱和蛋白质数据库搜索进行确认。共鉴定到 39 种蛋白质，包括 18 种上调蛋白和 21 种下调蛋白，分为不同类别，如能量和物质代谢、蛋白质翻译、次生代谢、植物激素、细胞骨架结构、调节和应激耐受。其中，最大的功能类别与初级代谢有关。下调蛋白参与光合作用、转录和翻译，而上调蛋白参与呼吸作用。类黄酮合成减少和 GA20ox 的上调可能参与了花逆转过程。上调的 14-3-3 蛋白通过响应非生物胁迫在龙眼花逆转的调节中发挥作用。透射电子显微镜观察显示了正在经历花逆转的脱落芽的超微结构变化。总的来说，这些结果为龙眼花逆转机制的分子基础提供了深入了解。
