Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry and Department of Chemistry, Lanzhou University, Lanzhou, PR China.
Int J Biol Macromol. 2012 Jan 1;50(1):250-6. doi: 10.1016/j.ijbiomac.2011.11.002. Epub 2011 Nov 9.
This study was carried out to develop a new type of modified starch based on α-amylase and glucoamylase. The structural and chemical characteristics of the porous starch were determined by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetry (DSC). The potential application of the porous starch as an adsorbent was evaluated using methyl violet as an adsorbed model. The adsorption capacity was optimized by investigating the reaction factors, including the mass ratio of α-amylase to glucoamylase (m(α-amylase)/m(glucoamylase)), the mass ratio of total amount of enzymes to starch (m(enzyme)/m(St)), the ratio of liquid volume to starch mass (V(H2O)/M(St)), pH value of the reaction solution, enzymatic reaction temperature, and enzymatic reaction time. The hydrolysis ratio of each sample was also determined to investigate the effect of different reaction conditions on the hydrolysis degree. The results suggest that the porous starch has a more excellent adsorption capacity than the native starch, and may be expected to have wide potential applications in many fields.
本研究旨在开发一种新型的基于α-淀粉酶和糖化酶的改性淀粉。通过傅里叶变换红外（FTIR）光谱、扫描电子显微镜（SEM）、X 射线衍射（XRD）和差示扫描量热法（DSC）测定多孔淀粉的结构和化学特性。使用甲基紫作为吸附模型评估多孔淀粉作为吸附剂的潜在应用。通过考察反应因素，包括α-淀粉酶与糖化酶的质量比（m(α-amylase)/m(glucoamylase)）、酶总量与淀粉的质量比（m(enzyme)/m(St)）、液固比（V(H2O)/M(St)）、反应溶液的 pH 值、酶反应温度和酶反应时间，优化了吸附容量。还测定了每个样品的水解率，以研究不同反应条件对水解程度的影响。结果表明，多孔淀粉具有比天然淀粉更优异的吸附能力，有望在许多领域得到广泛应用。
