Institute of Agrochemistry and Food Technology (IATA-CSIC), Avenida Agustin Escardino, 7, Paterna 46980, Valencia, Spain.
Carbohydr Polym. 2014 Jan 30;101:837-45. doi: 10.1016/j.carbpol.2013.10.013. Epub 2013 Oct 12.
Porous starch is attracting very much attention for its absorption and shielding ability in many food applications. The effect of two different enzymes, fungal α-amylase (AM) or amyloglucosidase (AMG), on corn starch at sub-gelatinization temperature was studied as an alternative to obtain porous starch. Biochemical features, thermal and structural analyses of treated starches were studied. Microscopic analysis of the granules confirmed the enzymatic modification of the starches obtaining porous structures with more agglomerates in the case of AMG treated starches. Several changes in thermal properties and hydrolysis kinetics were observed in enzymatically modified starches. Hydration properties were significantly affected by enzymatic modification being greater influenced by AMG activity, and the opposite trend was observed in the pasting properties. Overall, results showed that enzymatic modification at sub-gelatinization temperatures really offer an attractive alternative for obtaining porous starch granules to be used in a variety of foods applications.
多孔淀粉因其在许多食品应用中的吸收和屏蔽能力而备受关注。本文研究了两种不同的酶,真菌α-淀粉酶(AM)或糖化酶(AMG),在低于糊化温度下对玉米淀粉的作用,以期获得多孔淀粉。对处理后的淀粉进行了生化特性、热特性和结构分析。颗粒的微观分析证实了淀粉的酶改性,用 AMG 处理的淀粉获得了具有更多团聚体的多孔结构。在酶改性淀粉中观察到热性质和水解动力学的多种变化。水合性质受酶改性的显著影响,AMG 活性的影响更大,而在糊化性质中则观察到相反的趋势。总的来说,结果表明,低于糊化温度的酶改性确实为获得多孔淀粉颗粒提供了一种有吸引力的替代方法,可用于各种食品应用。