William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave., Columbus, OH 43210, USA.
Bioresour Technol. 2012 Jan;104:380-7. doi: 10.1016/j.biortech.2011.10.089. Epub 2011 Nov 2.
Concentrated cassava bagasse hydrolysate (CBH) containing 584.4 g/L glucose was studied for acetone-butanol-ethanol (ABE) fermentation with a hyper-butanol-producing Clostridium acetobutylicum strain in a fibrous bed bioreactor with gas stripping for continuous butanol recovery. With periodical nutrient supplementation, stable production of n-butanol from glucose in the CBH was maintained in the fed-batch fermentation over 263 h with an average sugar consumption rate of 1.28 g/L h and butanol productivity of 0.32±0.03 g/L h. A total of 108.5 g/L ABE (butanol: 76.4 g/L, acetone: 27.0 g/L, ethanol: 5.1 g/L) was produced, with an overall yield of 0.32±0.03 g/g glucose for ABE and 0.23±0.01 g/g glucose for butanol. The gas stripping process generated a product containing 10-16% (w/v) of butanol, ~4% (w/v) of acetone, a small amount of ethanol (<0.8%) and almost no acids, resulting in a highly concentrated butanol solution of ~64% (w/v) after phase separation.
研究了含有 584.4g/L 葡萄糖的浓缩木薯渣水解液（CBH），采用带气体汽提的纤维床生物反应器，以产超高丁醇梭菌 Clostridium acetobutylicum 为发酵菌株，进行丙酮丁醇乙醇（ABE）发酵，连续回收丁醇。通过周期性补料，在 263h 的补料分批发酵中，以 CBH 中的葡萄糖为底物，实现了稳定的丁醇生产，平均糖消耗速率为 1.28g/L·h，丁醇生产力为 0.32±0.03g/L·h。共生产了 108.5g/L 的 ABE（丁醇：76.4g/L，丙酮：27.0g/L，乙醇：5.1g/L），ABE 的总得率为 0.32±0.03g/g 葡萄糖，丁醇的得率为 0.23±0.01g/g 葡萄糖。气体汽提过程生成了一种含有 10-16%（w/v）丁醇、~4%（w/v）丙酮、少量乙醇（<0.8%）和几乎不含酸的产品，经相分离后得到约 64%（w/v）的高浓度丁醇溶液。
