College of Bioengineering, Chongqing University, Chongqing, China.
Acta Pharmacol Sin. 2012 Feb;33(2):237-41. doi: 10.1038/aps.2011.146. Epub 2011 Nov 21.
To explore the role of the glucagon-like peptide 1 receptor (GLP-1R) in geniposide regulated insulin secretion in rat INS-1 insulinoma cells.
Rat INS-1 insulinoma cells were cultured. The content of insulin in the culture medium was measured with ELISA assay. GLP-1R gene in INS-1 cells was knocked down with shRNA interference. The level of GLP-1R protein in INS-1 cells was measured with Western blotting.
Geniposide (0.01-100 μmol/L) increased insulin secretion from INS-1 cells in a concentration-dependent manner. Geniposide (10 μmol/L) enhanced acute insulin secretion in response to both the low (5.5 mmol/L) and moderately high levels (11 mmol/L) of glucose. Blockade of GLP-1R with the GLP-1R antagonist exendin (9-39) (200 nmol/L) or knock-down of GLP-1R with shRNA interference in INS-1 cells decreased the effect of geniposide (10 μmol/L) on insulin secretion stimulated by glucose (5.5 mmol/L).
Geniposide increases insulin secretion through glucagon-like peptide 1 receptors in rat INS-1 insulinoma cells.
探讨胰高血糖素样肽 1 受体（GLP-1R）在栀子苷调节大鼠 INS-1 胰岛细胞瘤细胞胰岛素分泌中的作用。
培养大鼠 INS-1 胰岛细胞瘤细胞。采用 ELISA 法检测培养基中胰岛素的含量。用 shRNA 干扰技术敲低 INS-1 细胞中的 GLP-1R 基因。用 Western blot 法测定 INS-1 细胞中 GLP-1R 蛋白的水平。
栀子苷（0.01-100 μmol/L）呈浓度依赖性地增加 INS-1 细胞的胰岛素分泌。栀子苷（10 μmol/L）增强了对低（5.5 mmol/L）和中高（11 mmol/L）葡萄糖水平的急性胰岛素分泌反应。用 GLP-1R 拮抗剂 exendin（9-39）（200 nmol/L）或 shRNA 干扰技术敲低 INS-1 细胞中的 GLP-1R，均可降低栀子苷（10 μmol/L）对葡萄糖（5.5 mmol/L）刺激的胰岛素分泌的作用。
栀子苷通过大鼠 INS-1 胰岛细胞瘤细胞中的胰高血糖素样肽 1 受体增加胰岛素分泌。
