Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA.
Nanotechnology. 2011 Dec 9;22(49):494008. doi: 10.1088/0957-4484/22/49/494008. Epub 2011 Nov 21.
This paper describes an efficient and versatile method for the fabrication of nanostructured substrates from a piece of tendon which comprises aligned collagen nanofibers. We used a microtome to generate the tendon slices (10-50 µm thick), which were used as a scaffold for guiding directional cell growth. Highly aligned and uniform monolayer cells sheets were obtained. The tendon slices were used as a master, and the nanostructures outlined by the bundles of collagen nanofibers were successfully transferred onto a polystyrene film using standard soft lithography. The cell growing on the nanostructured polystyrene substrate showed good adhesion and alignment. The technique developed here enables one to fabricate nanostructured substrates without using any traditional micro/nanofabrication tools. The nanostructured substrate, e.g. a slice of tendon, has excellent biocompatibility and relatively good mechanical stability, which makes this technique useful in constructing complicated 3D tissues.
本文描述了一种从包含定向胶原纳米纤维的肌腱片制造纳米结构基底的高效、通用的方法。我们使用切片机生成肌腱片（10-50 μm 厚），将其用作引导定向细胞生长的支架。获得了高度定向和均匀的单层细胞片。肌腱片用作主模板，通过胶原纳米纤维束勾勒出的纳米结构成功地通过标准软光刻转移到聚苯乙烯薄膜上。在纳米结构化聚苯乙烯基底上生长的细胞表现出良好的粘附性和定向性。这里开发的技术使得可以在不使用任何传统的微/纳米制造工具的情况下制造纳米结构基底。纳米结构基底，例如肌腱片，具有优异的生物相容性和相对较好的机械稳定性，这使得该技术在构建复杂的 3D 组织中非常有用。
