Biomedical Engineering Program, University of South Dakota, 4800 North Career Avenue, Sioux Falls, SD 57107, USA.
Macromol Biosci. 2011 Mar 10;11(3):391-9. doi: 10.1002/mabi.201000352. Epub 2010 Nov 15.
The annulus fibrosus comprises concentric lamellae that can be damaged due to intervertebral disc degeneration; to provide permanent repair of these acquired structural defects, one solution is to fabricate scaffolds that are designed to support the growth of annulus fibrosus cells. In this study, electrospun nanofibrous scaffolds of polycaprolactone are fabricated in random, aligned, and round-end configurations. Primary porcine annulus fibrosus cells are grown on the scaffolds and evaluated for attachment, proliferation, and production of extracellular matrix. The scaffold consisting of round-end nanofibers substantially outperforms the random and aligned scaffolds on cell adhesion; additionally, the scaffold with aligned nanofibers strongly affects the orientation of cells.
纤维环由同心层组成，可因椎间盘退变而受损；为了永久性修复这些获得性结构缺陷，一种解决方案是制造支架，旨在支持纤维环细胞的生长。在这项研究中，制备了聚己内酯的随机、定向和圆端结构的电纺纳米纤维支架。将原代猪纤维环细胞种植在支架上，并对其附着、增殖和细胞外基质的产生进行评估。由圆端纳米纤维组成的支架在细胞黏附方面明显优于随机和定向支架；此外，具有定向纳米纤维的支架强烈影响细胞的取向。
