超分辨率光场波动成像（SOFI）。

Superresolution optical fluctuation imaging (SOFI).

机构信息

Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.

出版信息

Adv Exp Med Biol. 2012;733:17-21. doi: 10.1007/978-94-007-2555-3_2.

Abstract

Superresolution microscopy has shifted the limits for fluorescence microscopy in cell -biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed "superresolution optical fluctuation -imaging" (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.

摘要

超分辨率显微镜已经改变了细胞生物学中荧光显微镜的限制。在固定甚至活细胞和生物体中以几纳米的分辨率成像细胞结构和动态的可能性为未来的生物学发现带来了巨大的希望。我们最近引入了一种新的超分辨率技术，该技术基于对源自单个发射器的随机波动的统计评估，称为“超分辨率光波动成像”（SOFI）。与以前介绍的超分辨率方法相比，SOFI 具有简单、经济、高速和低光暴露水平等优点。在这里，我们总结了基本的工作原理和最新进展。