Institut d'Electronique Fondamentale, Université Paris-Sud, Orsay, France.
Adv Exp Med Biol. 2012;733:75-86. doi: 10.1007/978-94-007-2555-3_8.
Applications based on Förster resonance energy transfer (FRET) play an important role for the determination of concentrations and distances within nanometer-scale systems in vitro and in vivo in many fields of biotechnology. Semiconductor nanocrystals (Quantum dots - QDs) possess ideal properties for their application as FRET acceptors when the donors have long excited state lifetimes and when direct excitation of QDs can be efficiently suppressed. Therefore, luminescent terbium complexes (LTCs) with excited state lifetimes of more than 2 ms are ideal FRET donor candidates for QD-acceptors. This chapter will give a short overview of theoretical and practical background of FRET, QDs and LTCs, and present some recent applications of LTC-QD FRET pairs for multiplexed ultra-sensitive in vitro diagnostics and nanometer-resolution molecular distance measurements.
基于Förster 共振能量转移（FRET）的应用在生物技术的许多领域中，对于体外和体内纳米级系统中浓度和距离的确定起着重要作用。半导体纳米晶体（量子点-QD）具有作为 FRET 受体的理想特性，当供体具有长激发态寿命并且可以有效地抑制 QD 的直接激发时。因此，具有超过 2 ms 的激发态寿命的发光铽配合物（LTC）是 QD-受体的理想 FRET 供体候选物。本章将简要概述 FRET、QD 和 LTC 的理论和实践背景，并介绍 LTC-QD FRET 对用于多重超灵敏体外诊断和纳米分辨率分子距离测量的一些最新应用。
