来自铜绿假单胞菌的一种假定芳香酸脱羧酶PA4019的结构
Structure of PA4019, a putative aromatic acid decarboxylase from Pseudomonas aeruginosa.
作者信息
Kopec Jolanta, Schnell Robert, Schneider Gunter
机构信息
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Oct 1;67(Pt 10):1184-8. doi: 10.1107/S174430911102923X. Epub 2011 Sep 24.
Abstract
The ubiX gene (PA4019) of Pseudomonas aeruginosa has been annotated as encoding a putative 3-octaprenyl-4-hydroxybenzoate decarboxylase from the ubiquinone-biosynthesis pathway. Based on a transposon mutagenesis screen, this gene was also implicated as being essential for the survival of this organism. The crystal structure of recombinant UbiX determined to 1.5 Å resolution showed that the protein belongs to the superfamily of homo-oligomeric flavine-containing cysteine decarboxylases. The enzyme assembles into a dodecamer with 23 point symmetry. The subunit displays a typical Rossmann fold and contains one FMN molecule bound at the interface between two subunits.
摘要
铜绿假单胞菌的ubiX基因（PA4019）已被注释为编码一种来自泛醌生物合成途径的推定3-辛基-4-羟基苯甲酸脱羧酶。基于转座子诱变筛选，该基因也被认为对该生物体的存活至关重要。确定分辨率为1.5 Å的重组UbiX晶体结构表明，该蛋白质属于含黄素的同型寡聚半胱氨酸脱羧酶超家族。该酶组装成具有23点对称性的十二聚体。亚基呈现典型的罗斯曼折叠，并且在两个亚基之间的界面处含有一个结合的FMN分子。
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