Department of Clinical Neurophysiology, University Medical Centre Göttingen, Göttingen, Germany.
Muscle Nerve. 2011 Dec;44(6):930-6. doi: 10.1002/mus.22210.
To test the hypothesis that the efficacy of botulinum toxin depends on the activity of the neuromuscular junction, we developed an in vivo paradigm to determine the degree and duration of low-dose botulinum toxin-induced focal paresis in mice.
We combined an automated wheel-running paradigm with low-dose botulinum toxin injections into the calf muscles of wild-type mice. Half of the mice were injected either before the nightly running or before the daily resting period.
After botulinum toxin injections, running distance and maximum velocity decreased dose-dependently. The degree and duration of decrease between the respective groups with regard to the time-points of injection were identical.
This in vivo paradigm quantifies the degree of otherwise clinically inapparent botulinum toxin-induced focal calf muscle paresis. Increased muscle activity after low-dose injections does not influence the efficacy of botulinum toxin in normal muscles.
为了验证肉毒毒素的疗效取决于运动终板活性这一假说，我们开发了一种体内范例，以确定在小鼠中低剂量肉毒毒素诱导的局灶性瘫痪的程度和持续时间。
我们将自动轮跑范例与小腿肌肉的低剂量肉毒毒素注射结合起来，应用于野生型小鼠。一半的小鼠在夜间跑步前或每日休息前接受注射。
肉毒毒素注射后，跑步距离和最大速度呈剂量依赖性下降。各注射时间点的组之间下降的程度和持续时间相同。
这种体内范例定量了临床上无明显的肉毒毒素诱导的局灶性小腿肌肉瘫痪的程度。低剂量注射后肌肉活动增加不会影响正常肌肉中肉毒毒素的疗效。
