Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan.
J Orthop Res. 2013 Jan;31(1):125-31. doi: 10.1002/jor.22180. Epub 2012 Jun 25.
Treadmill running is a commonly used training method for patients with spasticity to improve functional performance. Botulinum toxin has been widely used therapeutically to reduce contraction force of spastic muscle. However, the effects of treadmill running in neuromuscular junction expression and motor unit physiology on muscle following botulinum toxin injection are not well established. To assess the effects of treadmill running on neuromuscular recovery of gastrocnemius following botulinum toxin A (BoNT-A) injection, we observed changes in gene expression. We hypothesized that the expression of acetylcholine receptor (AChR), myogenesis, and nerve plasticity could be enhanced. Twenty-four Sprague-Dawley rats received botulinum toxin injection in right gastrocnemius and were then randomly assigned into untrained control and treadmill running groups. The rats assigned to the treadmill running group were trained on a treadmill 3 times/week with a running speed of 15 m/min for 8 weeks. The duration of training was 20 min per session. Muscle strength and gene expression of AChR subunit (α, β, δ, γ, and ε), MyoD, Myf-5, MRF4, myogenin, p21, IGF-1, GAP43, were analyzed. Treadmill running had no influence on gastrocnemius mass, but improved the maximal contraction force of the gastrocnemius in the treadmill running group (p < 0.05). Upregulation of GAP-43, IGF-1, Myo-D, Myf-5, myogenin, and AChR subunits α and β were found following treadmill running. The expression of genes associated with neurite and AChR regeneration following treadmill exercise was upregulated, which may have contributed to enhanced recovery of gastrocnemius strength.
跑步机跑步是一种常用于改善痉挛患者功能表现的训练方法。肉毒毒素已广泛用于治疗以降低痉挛肌肉的收缩力。然而，在接受肉毒毒素注射后，跑步机跑步对运动终板表达和运动单位生理学的影响尚不清楚。为了评估跑步机跑步对肉毒毒素 A (BoNT-A) 注射后腓肠肌神经肌肉恢复的影响，我们观察了基因表达的变化。我们假设乙酰胆碱受体 (AChR)、成肌和神经可塑性的表达可以增强。24 只 Sprague-Dawley 大鼠在右侧腓肠肌接受肉毒毒素注射，然后随机分为未训练对照组和跑步机跑步组。分配到跑步机跑步组的大鼠每周在跑步机上训练 3 次，速度为 15m/min，持续 8 周。每次训练持续 20 分钟。分析肌肉力量和 AChR 亚基 (α、β、δ、γ 和 ε)、MyoD、Myf-5、MRF4、myogenin、p21、IGF-1、GAP43 的基因表达。跑步机跑步对腓肠肌质量没有影响，但提高了跑步机跑步组腓肠肌的最大收缩力（p<0.05）。跑步机跑步后发现 GAP-43、IGF-1、Myo-D、Myf-5、myogenin 和 AChR 亚基 α 和 β 的表达上调。与神经突和 AChR 再生相关的基因表达上调，这可能有助于增强腓肠肌力量的恢复。
