Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Kazan 420111, Russia.
Muscle Nerve. 2011 Dec;44(6):987-9. doi: 10.1002/mus.22250.
In this study we demonstrate expression of the N-methyl-D-aspartate receptor NR1 subunit in the rat neuromuscular junction of skeletal muscles of different functional types (extensor digitorum longus, soleus, and diaphragm muscles) using fluorescence immunocytochemistry. Electron microscopic immunocytochemistry has shown that the NR1 subunit is localized solely on the sarcolemma in the depths of the postsynaptic folds. These findings suggest participation of the glutamatergic signaling system in functioning of the adult mammalian neuromuscular junction.
在这项研究中，我们使用荧光免疫细胞化学技术证明了 N-甲基-D-天冬氨酸受体 NR1 亚基在不同功能类型（伸趾长肌、比目鱼肌和膈肌）的大鼠骨骼肌神经肌肉接头中的表达。电子显微镜免疫细胞化学显示，NR1 亚基仅位于突触后褶皱深处的肌膜上。这些发现表明，谷氨酸能信号系统参与了成年哺乳动物神经肌肉接头的功能。
