Bouia A, Bringel F, Frey L, Belarbi A, Guyonvarch A, Kammerer B, Hubert J C
Laboratoire de Microbiologie, Université Louis-Pasteur, Strasbourg, France.
FEMS Microbiol Lett. 1990 Jun 1;57(3):233-8. doi: 10.1016/0378-1097(90)90072-x.
The pyrE gene of Lactobacillus plantarum CCM 1904, coding for the orotate phosphoribosyl transferase involved in the pyrimidine biosynthetic pathway, was cloned in Escherichia coli and sequenced. The predicted polypeptide sequence extending over 212 amino acids (MW 22,690) was compared to those of E. coli and to those of lower eukaryotes (Saccharomyces cerevisiae, Podospora anserina, Sordaria macrospora, Dictyostelium discoideum). Important conserved stretches were revealed, implying that these proteins are closely related.
植物乳杆菌CCM 1904的pyrE基因编码参与嘧啶生物合成途径的乳清酸磷酸核糖基转移酶，该基因在大肠杆菌中克隆并测序。将预测的由212个氨基酸组成（分子量22,690）的多肽序列与大肠杆菌以及低等真核生物（酿酒酵母、嗜热栖热放线菌、大孢粪壳菌、盘基网柄菌）的序列进行比较。发现了重要的保守序列，这表明这些蛋白质密切相关。
