de Montigny J, Belarbi A, Hubert J C, Lacroute F
Laboratoire de Génétique Physiologique, I.B.M.C. du C.N.R.S., Strasbourg, France.
Mol Gen Genet. 1989 Feb;215(3):455-62. doi: 10.1007/BF00427043.
The URA5 gene of Saccharomyces cerevisiae encodes orotate phosphoribosyl transferase (EC 2.4.2.10; OPRTase) which catalyses the transformation of orotate to OMP in the pyrimidine pathway. We present in this paper the cloning and the sequencing of this gene, the last in the yeast pyrimidine pathway to be cloned. We have deduced the protein sequence of the OPRTase of S. cerevisiae from the DNA sequence and compared it to that of Escherichia coli, Podospora anserina and Dictyostelium discoideum. Some important similarities in the structure of these four proteins have been found. Finally, we have quantified the transcription of the URA5 gene in different physiological conditions and confirmed that it was not under the control of UTP or any intermediary product of the pathway.
酿酒酵母的URA5基因编码乳清酸磷酸核糖转移酶（EC 2.4.2.10；OPRTase），该酶在嘧啶途径中催化乳清酸转化为OMP。我们在本文中展示了该基因的克隆和测序，这是酵母嘧啶途径中最后一个被克隆的基因。我们从DNA序列推导了酿酒酵母OPRTase的蛋白质序列，并将其与大肠杆菌、嗜鸟粪盘菌和盘基网柄菌的序列进行了比较。发现这四种蛋白质在结构上有一些重要的相似之处。最后，我们定量了URA5基因在不同生理条件下的转录，并证实其不受UTP或该途径任何中间产物的调控。
