Center for Regenerative Medicine and Marriott Heart Disease Research Program, MN, USA.
Biomark Med. 2011 Dec;5(6):715-29. doi: 10.2217/bmm.11.87.
Nuclear reprogramming with stemness factors enables resetting of somatic differentiated tissue back to the pluripotent ground state. Recent evidence implicates mitochondrial restructuring and bioenergetic plasticity as key components underlying execution of orchestrated dedifferentiation and derivation of induced pluripotent stem cells. Aerobic to anaerobic transition of somatic oxidative energy metabolism into a glycolytic metabotype promotes proficient reprogramming, establishing a novel regulator of acquired stemness. Metabolomic profiling has further identified specific metabolic remodeling traits defining lineage redifferentiation of pluripotent cells. Therefore, mitochondrial biogenesis and energy metabolism comprise a vital axis for biomarker discovery, intimately reflecting the molecular dynamics fundamental for the resetting and redirection of cell fate.
利用干性因子进行核重编程可将体细胞核重新诱导回到多能状态。最近的证据表明，线粒体重构和生物能量可塑性是协调去分化和诱导多能干细胞衍生的关键组成部分。体细胞氧化能量代谢从需氧型向糖酵解代谢类型的无氧转变促进了有效的重编程，为获得的干性建立了新的调控因子。代谢组学分析进一步确定了定义多能细胞谱系再分化的特定代谢重塑特征。因此，线粒体生物发生和能量代谢构成了生物标志物发现的重要轴，它密切反映了重置和重定向细胞命运的基本分子动力学。
