Kaur M, Gupta M, Tripathi K K, Gupta K G
Department of Microbiology, Panjab University, Chandigarh, India.
Folia Microbiol (Praha). 1990;35(3):183-9. doi: 10.1007/BF02820483.
Elastase of Vibrio cholerae caused the lysis of freshly grown cells of Gram-negative (Pseudomonas aeruginosa, Proteus vulgaris, Salmonella paratyphi A and Klebsiella pneumoniae) bacteria. Gram-positive (Staphylococcus aureus and S. epidermidis) organisms were resistant to this enzyme. Heat killed and lyophilized Gram-positive and -negative bacteria (except S. aureus and S. epidermidis) showed higher sensitivity to elastase. Both Gram-negative and -positive bacteria were lyzed maximally by elastase at pH 8.0. At this pH, lytic activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers.
霍乱弧菌弹性蛋白酶可导致革兰氏阴性菌（铜绿假单胞菌、普通变形杆菌、副伤寒沙门氏菌A和肺炎克雷伯菌）新鲜培养细胞的裂解。革兰氏阳性菌（金黄色葡萄球菌和表皮葡萄球菌）对这种酶具有抗性。经加热杀死并冻干的革兰氏阳性和阴性细菌（金黄色葡萄球菌和表皮葡萄球菌除外）对弹性蛋白酶表现出更高的敏感性。弹性蛋白酶在pH 8.0时对革兰氏阴性和阳性细菌的裂解作用最大。在此pH值下，弹性蛋白酶在Tris-HCl和甘氨酸-NaOH缓冲液中的裂解活性最高，其次是Tris-马来酸盐和二甲胂酸盐缓冲液。
