Gupta M, Kaur M, Gupta K G
Department of Microbiology, Panjab University, Chandigarh, India.
Indian J Exp Biol. 1992 May;30(5):380-3.
Elastase of B. subtilis 6a caused lysis of freshly grown cells of Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, Salmonella typhi and Pseudomonas aeruginosa and Gram-positive (B. subtilis) bacteria. Heat killed and lyophilised Gram-positive and negative bacteria showed higher sensitivity to elastase. Both Gram-negative and Gram-positive bacteria were lysed maximally by elastase at pH 8.0. At this pH, activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers.
枯草芽孢杆菌6a的弹性蛋白酶可导致革兰氏阴性菌（普通变形杆菌、肺炎克雷伯菌、伤寒沙门氏菌和铜绿假单胞菌）以及革兰氏阳性菌（枯草芽孢杆菌）的新鲜培养细胞发生裂解。经加热灭活和冻干处理的革兰氏阳性菌和阴性菌对弹性蛋白酶表现出更高的敏感性。弹性蛋白酶在pH 8.0时对革兰氏阴性菌和革兰氏阳性菌的裂解作用最强。在此pH值下，弹性蛋白酶在Tris-HCl和甘氨酸-NaOH缓冲液中的活性最高，其次是Tris-马来酸缓冲液和二甲胂酸盐缓冲液。
