Kaur M, Tripathi K K, Gupta M, Jain P K, Bansal M R, Gupta K G
Department of Microbiology, Panjab University, Chandigarh, India.
Can J Microbiol. 1988 Jul;34(7):855-9. doi: 10.1139/m88-147.
Conditions are described for the production of extracellular elastase by Bacillus subtilis. The yield of enzyme was maximum in shake-cultures grown in Syncase medium at 37 degrees C and was stable in culture supernatants. The enzyme, purified by ammonium sulphate precipitation and Sephadex G-75 gel filtration, showed a molecular weight of 25,000 and activity between pH 6.0 and 9.5, with an optimum of 9.0 in Tris-maleate buffer. Elastinolytic activity was maximum in glycine-NaOH buffer and minimum in phosphate buffer. Enzyme activity was adversely affected by temperature greater than or equal to 40 degrees C.
描述了枯草芽孢杆菌产生细胞外弹性蛋白酶的条件。在37℃下于Syncase培养基中进行摇瓶培养时，酶产量最高，且在培养上清液中稳定。通过硫酸铵沉淀和Sephadex G - 75凝胶过滤纯化的酶，分子量为25,000，在pH 6.0至9.5之间具有活性，在Tris - 马来酸缓冲液中的最适pH为9.0。弹性蛋白分解活性在甘氨酸 - NaOH缓冲液中最高，在磷酸盐缓冲液中最低。温度大于或等于40℃时，酶活性受到不利影响。
