L-色氨酸的酶促合成
Enzyme synthesis of L-tryptophan.
作者信息
Zeman R, Plachý J, Bulantová H, Sikyta B, Pavlasová E, Stejskalová E
机构信息
Research Institute of Antibiotics and Biotransformations, Roztoky near Prague, Czechoslovakia.
出版信息
Folia Microbiol (Praha). 1990;35(3):200-4. doi: 10.1007/BF02820485.
PMID:2210490
Abstract
Enzyme synthesis of tryptophan from indole, pyruvate and ammonium salts was studied using Escherichia coli cells exhibiting a significant tryptophanase activity. In addition to the effect of cultivation medium composition and cultivation conditions, factors affecting the course of the conversion were investigated. Production of 32.4 g/L of L-tryptophan was reached after 48 h under optimal conditions.
摘要
利用具有显著色氨酸酶活性的大肠杆菌细胞，研究了从吲哚、丙酮酸和铵盐酶促合成色氨酸的过程。除了培养基组成和培养条件的影响外，还研究了影响转化过程的因素。在最佳条件下，48小时后L-色氨酸产量达到32.4 g/L。
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