Cristea Violeta-Corina, Duţă Maria, Neacşu Gabriela
Synevo Laboratory, Bucharest, Romania.
Roum Arch Microbiol Immunol. 2011 Apr-Jun;70(2):65-8.
We examined group B streptococcus (GBS) isolates colonizing women at the 35-37 weeks of pregnancy. A total of 257 group B streptococcus (GBS) isolates for serotyped using direct agglutination with a set of commercially available antisera (Ia, Ib, II, III, IV, and V) and tested for susceptibility to antimicrobials (penicillin, macrolides, lincosamides, fluoroquinolones and tetracyclines). Fourteen isolates could not be serotyped with the antisera set used in the study. Serotype III was the predominant serotype (33%), followed by serotypes V (23%), and Ia (20%). Whereas all isolates were susceptible to penicillin, the rates of susceptibility to the other antimicrobials tested were the following: 91% for ofloxacin, 80% for clindamycin, 77% for erythromycin, and 4% for tetracycline. More than half (67%) of the macrolide resistant isolates belonged to serotypes V and III. A systematic surveillance of the autochthonous GBS serotypes, performed at the level of laboratories processing a high number of human specimens, is mandatory for strengthening the national epidemiological GBS surveillance. While penicillin remains the drug of choice for intrapartum prophylaxis, the resistance of autochthonous GBS isolates to other antibiotics should be actively monitored.
我们检测了在妊娠35至37周时定植于女性体内的B族链球菌（GBS）分离株。总共257株B族链球菌（GBS）分离株使用一组市售抗血清（Ia、Ib、II、III、IV和V）通过直接凝集法进行血清分型，并检测其对抗微生物药物（青霉素、大环内酯类、林可酰胺类、氟喹诺酮类和四环素类）的敏感性。14株分离株无法用本研究中使用的抗血清进行血清分型。血清型III是主要血清型（33%），其次是血清型V（23%）和Ia（20%）。所有分离株对青霉素均敏感，而对其他检测抗菌药物的敏感率如下：氧氟沙星为91%，克林霉素为80%，红霉素为77%，四环素为4%。超过一半（67%）的大环内酯类耐药分离株属于血清型V和III。在处理大量人类标本的实验室层面开展对本地GBS血清型的系统监测，对于加强国家GBS流行病学监测至关重要。虽然青霉素仍然是产时预防的首选药物，但应积极监测本地GBS分离株对其他抗生素的耐药情况。
