Giannitsis D J, Flierl D, Schuler G, Häcker-Shahin B
Abt. f. klin. Hämostaseologie u. Transfusionsmedizin der Universitätskliniken Homburg/Saar.
Infusionstherapie. 1990 Aug;17(4):217-9. doi: 10.1159/000222484.
A micro enzyme-linked immunosorbent assay (ELISA), using lyophilized stroma as carrier of red blood cell antigens, which stay stable longer than usual, using intact erythrocytes, was developed for determination of blood-group antibodies in the AB0, Kell and Lewis-systems. Stroma being fixed on microtiter plates was incubated with antisera and peroxidase-conjugated anti-human globulin. The transformation of the substrate added was determined photometrically. A binding of antibodies to the stroma could be demonstrated up to an antibody dilution of 1:1024 for the ABO-system, of 1:512 for the Kell-system and of 1:64 for the Lewis-system. By standardization of this method the quantitative determination of antibodies becomes possible without being restricted by the limited stability of intact erythrocytes.
一种微量酶联免疫吸附测定法（ELISA）被开发出来用于测定ABO、凯尔（Kell）和刘易斯（Lewis）血型系统中的血型抗体。该方法使用冻干的基质作为红细胞抗原的载体，其稳定性比通常情况更长，且使用完整红细胞。固定在微量滴定板上的基质与抗血清和过氧化物酶标记的抗人球蛋白一起孵育。通过光度法测定添加底物的转化情况。对于ABO血型系统，抗体与基质的结合在抗体稀释至1:1024时仍可被检测到；对于凯尔血型系统，在稀释至1:512时可被检测到；对于刘易斯血型系统，在稀释至1:64时可被检测到。通过该方法的标准化，无需受完整红细胞有限稳定性的限制即可实现抗体的定量测定。
