Ogiso M, Saito N, Sudo K, Kubo H, Hirano S, Komoto M
Department of Physiology, Toho University School of Medicine, Tokyo, Japan.
Invest Ophthalmol Vis Sci. 1990 Oct;31(10):2171-9.
Gangliosides were isolated from human senile cataractous lenses by solvent extraction, DEAE-Sephadex column chromatography, and thin-layer chromatography. The content and composition of gangliosides were examined in individual lens tissues. Three predominant gangliosides, GM3, GM1, and GD1a, were tentatively identified in comparison with authentic brain gangliosides, and several unidentified gangliosides were also recognized. The increase in ganglioside content per mg of protein content in cataractous lenses was found to be influenced by two physiologic parameters: aging and cataract progression. The mature cataractous lenses showed a higher ganglioside level on a protein basis than the immature lenses compared with the same age group. On the basis of statistical analysis, an age-dependent increase in ganglioside concentration was recognized in both mature and immature lens groups. The relative increase in slow-moving polysialogangliosides on thin-layer chromatography seemed to be caused by the maturation of cataract. The sugar composition of one of the polysialogangliosides was found to be glucose, galactose, and sialic acid in the molar ratio of 2:1:4; this suggests the presence of a unique ganglioside species in human cataractous lens.
通过溶剂萃取、DEAE-葡聚糖凝胶柱色谱法和薄层色谱法从人老年性白内障晶状体中分离出神经节苷脂。对单个晶状体组织中的神经节苷脂含量和组成进行了检测。与正宗脑源性神经节苷脂相比，初步鉴定出三种主要的神经节苷脂，即GM3、GM1和GD1a，同时还识别出几种未鉴定的神经节苷脂。发现每毫克蛋白质含量的白内障晶状体中神经节苷脂含量的增加受两个生理参数影响：衰老和白内障进展。与同一年龄组相比，成熟白内障晶状体在蛋白质基础上的神经节苷脂水平高于未成熟晶状体。基于统计分析，在成熟和未成熟晶状体组中均发现神经节苷脂浓度随年龄增长而增加。薄层色谱上慢速移动的多唾液酸神经节苷脂的相对增加似乎是由白内障成熟引起的。发现其中一种多唾液酸神经节苷脂的糖组成为葡萄糖、半乳糖和唾液酸，摩尔比为2:1:4；这表明人白内障晶状体中存在一种独特的神经节苷脂种类。
