Ogiso M, Okinaga T, Ohta M, Komoto M, Hoshi M
PRESTO, Research Development Corporation of Japan (JRDC), Tokyo.
Biochim Biophys Acta. 1995 May 17;1256(2):166-74. doi: 10.1016/0005-2760(95)00027-a.
Human lens accumulates gangliosides in association with aging and senile cataract progression. In this study we purified and characterized five major gangliosides in human cataractous lenses. Structural analyses and immunological studies revealed the presence of ganglio-series gangliosides, GM3, GM2, GM1 and GD1a, and a sialyl-Lewisx-containing neolacto-series ganglioside, NeuAc alpha 2-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1ceramide (IV3NeuAcIII3FucnLc4). Slow-moving gangliosides, although minor components, were also found to have sialyl-Lewisx-related structures, based on anti-Lewisx antiserum binding to their asialo forms. However, sialyl-paragloboside, a possible precursor of the sialyl-Lewisx ganglioside, was not identified.
随着年龄增长和老年性白内障的发展，人晶状体中神经节苷脂会逐渐积累。在本研究中，我们对人白内障晶状体中的五种主要神经节苷脂进行了纯化和表征。结构分析和免疫学研究表明，存在神经节系列神经节苷脂GM3、GM2、GM1和GD1a，以及一种含唾液酸化路易斯x的新乳糖系列神经节苷脂，即NeuAcα2-3Galβ1-4（Fucα1-3）GlcNAcβ1-3Galβ1-4Glcβ1-1神经酰胺（IV3NeuAcIII3FucnLc4）。基于抗路易斯x抗血清与其脱唾液酸形式的结合，发现迁移缓慢的神经节苷脂尽管是次要成分，但也具有与唾液酸化路易斯x相关的结构。然而，未鉴定出唾液酸化副球蛋白，它可能是唾液酸化路易斯x神经节苷脂的前体。
