Fascin 促进胰腺癌细胞的运动性和侵袭性。
Fascin promotes the motility and invasiveness of pancreatic cancer cells.
机构信息
Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, Beijing 100730, China.
出版信息
World J Gastroenterol. 2011 Oct 28;17(40):4470-8. doi: 10.3748/wjg.v17.i40.4470.
Abstract
AIM
To explore the role of actin-bundling protein, fascin during the progression of pancreatic cancer.
METHODS
The plasmid expressing human fascin-1 was stably transfected into the pancreatic cancer cell line MIA PaCa-2. The proliferation, cell cycle, motility, scattering, invasiveness and organization of the actin filament system in fascin-transfected MIA PaCa-2 cells and control non-transfected cells were determined.
RESULTS
Heterogeneous overexpression of fascin markedly enhanced the motility, scattering, and invasiveness of MIA PaCa-2 cells. However, overexpression of fascin had minimal effect on MIA PaCa-2 cell proliferation and cell cycle. In addition, cell morphology and organization of the actin filament system were distinctly altered in fascin overexpressed cells. When transplanted into BALB/c-nu mice, fascin-transfected pancreatic cancer cells developed solid tumors at a slightly slower rate, but these tumors displayed more aggressive behavior in comparison with control tumors.
CONCLUSION
Fascin promotes pancreatic cancer cell migration, invasion and scattering, thus contributes to the aggressive behavior of pancreatic cancer cells.
摘要
目的
探讨微丝束形成蛋白 fascin 在胰腺癌进展过程中的作用。
方法
将表达人 fascin-1 的质粒稳定转染入胰腺癌细胞系 MIA PaCa-2。检测 fascin 转染的 MIA PaCa-2 细胞和对照未转染细胞的增殖、细胞周期、运动性、分散性、侵袭性和肌动蛋白丝系统的组织。
结果
异质性过表达 fascin 显著增强了 MIA PaCa-2 细胞的运动性、分散性和侵袭性。然而，过表达 fascin 对 MIA PaCa-2 细胞的增殖和细胞周期几乎没有影响。此外，fascin 过表达细胞的细胞形态和肌动蛋白丝系统的组织明显改变。当将 fascin 转染的胰腺癌细胞移植到 BALB/c-nu 小鼠中时，肿瘤的生长速度略慢，但与对照肿瘤相比，这些肿瘤表现出更具侵袭性的行为。
结论
fascin 促进胰腺癌细胞的迁移、侵袭和分散，从而促进胰腺癌细胞的侵袭性行为。
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