Brain Disease Research Center, and Division of Standard Research, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
Evid Based Complement Alternat Med. 2012;2012:109796. doi: 10.1155/2012/109796. Epub 2011 Oct 29.
Neuropeptide Y (NPY), which is widely expressed in both the central and peripheral nervous systems, has an important role in a variety of biological fields. In this study, we analyzed the distribution of NPY polymorphisms in dampness-phlegm pattern and non-dampness-phlegm pattern in elderly Korean subjects with cerebral infarction (CI). A total of 1.097 subjects (498 normal subjects and 599 CI patients, including 198 with dampness-phlegm pattern and 401 with non-dampness-phlegm pattern) participated in this study. Genotyping for five SNPs (G-1484A, C-1471T, C-399T, A1201G, and C5325T) was conducted by primer extension. The results were statistically analyzed for genetic association of NPY-polymorphisms with normal versus dampness-phlegm pattern or non-dampness-phlegm pattern subjects. Among the five SNPs tested, the T allele of C-399T has a negative association with the dampness-phlegm pattern and is marked by a decrease in serum cholesterol levels. Furthermore, serum cholesterol levels were significantly higher in dampness-phlegm pattern patients than in non-dampness-phlegm pattern patients.In this study, for the first time, the association of NPY polymorphisms with pattern identification (PI) of traditional Korean medicine (TKM) was analyzed in a large CI patient population.
神经肽 Y（NPY）广泛表达于中枢和外周神经系统，在多种生物学领域发挥着重要作用。本研究分析了神经肽 Y 多态性在老年韩国脑梗死（CI）患者痰湿证和非痰湿证中的分布。共有 1097 名受试者（498 名正常受试者和 599 名 CI 患者，包括 198 名痰湿证患者和 401 名非痰湿证患者）参与了这项研究。通过引物延伸法对五个 SNP（G-1484A、C-1471T、C-399T、A1201G 和 C5325T）进行基因分型。对 NPY 多态性与正常与痰湿证或非痰湿证受试者的遗传关联进行了统计学分析。在检测的五个 SNP 中，C-399T 的 T 等位基因与痰湿证呈负相关，表现为血清胆固醇水平降低。此外，痰湿证患者的血清胆固醇水平明显高于非痰湿证患者。在这项研究中，首次在大型 CI 患者人群中分析了 NPY 多态性与传统韩国医学（TKM）的模式识别（PI）的关系。
