Medical Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
Evid Based Complement Alternat Med. 2012;2012:798131. doi: 10.1155/2012/798131. Epub 2012 Aug 8.
WNT10B has been indicated as a potential regulator of adipogenesis in vivo and in vitro models of obesity. In this study, we analyzed the distribution of WNT10B polymorphism in elderly Korean subjects with cerebral infarction (CI) and Yin Deficiency pattern and Non-Yin Deficiency pattern. A total of 630 CI patients, including 75 with Yin Deficiency pattern and 555 with Non-Yin Deficiency pattern, participated in this study. SNP (G-607C) genotyping was conducted by primer extension using TaqMan probe; five percent of subjects were regenotyped by direct sequencing to confirm the accuracy of the genotyping. The results were analyzed using a multiple logistic regression model to evaluate the genetic association between the G-607C variant and Yin Deficiency pattern. The frequency of the CC genotype of G-607C in the Yin Deficiency pattern group (29.33%) was significantly higher than that in the Non-Yin Deficiency pattern group (23.96%) (P = 0.0339 , OR = 2.005 (1.054-3.814)) in a recessive model. This is the first study to demonstrate an association between a WNT10B polymorphism and the Yin Deficiency pattern of traditional Korean medicine (TKM) in a CI patient population. These results suggest that G-607C might be used as a diagnostic genetic marker for Yin Deficiency pattern in stroke patients and in the development of personalized medical care.
WNT10B 已被证实为体内和肥胖体外模型中脂肪生成的潜在调节因子。在这项研究中，我们分析了 WNT10B 多态性在患有脑梗死 (CI) 和阴虚证及非阴虚证的老年韩国受试者中的分布。共有 630 名 CI 患者参与了本研究，其中 75 名患者为阴虚证，555 名患者为非阴虚证。采用 TaqMan 探针的引物延伸法进行 SNP（G-607C）基因分型；对 5%的受试者进行直接测序以重新进行基因分型，以确认基因分型的准确性。采用多因素逻辑回归模型分析 G-607C 变异与阴虚证之间的遗传关联。在隐性模型中，阴虚证组 G-607C 的 CC 基因型频率（29.33%）显著高于非阴虚证组（23.96%）（P=0.0339，OR=2.005[1.054-3.814]）。这是首次在 CI 患者人群中表明 WNT10B 多态性与传统韩国医学（TKM）阴虚证之间存在关联的研究。这些结果表明，G-607C 可能被用作中风患者阴虚证的诊断遗传标志物，并有助于开发个性化医疗。
