Jeffery Constance J
Department of Biological Sciences, Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, USA.
Nano Rev. 2011;2. doi: 10.3402/nano.v2i0.5743. Epub 2011 Jan 19.
Diabetes affects over 100 million people worldwide. Better methods for monitoring blood glucose levels are needed for improving disease management. Several labs have previously made glucose nanosensors by modifying members of the periplasmic ligand binding protein superfamily. This minireview summarizes recent developments in constructing new versions of these proteins that are responsive within the physiological range of blood glucose levels, employ new reporter groups, and/or are more robust. These experiments are important steps in the development of novel proteins that have the characteristics needed for an implantable glucose nanosensor for diabetes management: specificity for glucose, rapid response, sensitivity within the physiological range of glucose concentrations, reproducibility, and robustness.
糖尿病影响着全球超过1亿人。为了改善疾病管理，需要更好的血糖水平监测方法。此前，几个实验室通过修饰周质配体结合蛋白超家族的成员制备了葡萄糖纳米传感器。本综述总结了构建这些蛋白质新版本的最新进展，这些新版本在血糖水平的生理范围内具有响应性、采用了新的报告基团和/或更稳定。这些实验是开发新型蛋白质的重要步骤，这些蛋白质具有用于糖尿病管理的可植入葡萄糖纳米传感器所需的特性：对葡萄糖的特异性、快速响应、在葡萄糖浓度生理范围内的灵敏度、可重复性和稳定性。
