The University of Queensland, School of Medicine, Woolloongabba, Queensland, 4102, Australia.
J Biomed Opt. 2011 Nov;16(11):116011. doi: 10.1117/1.3647597.
Ischemia-reperfusion (I/R) injury is a common occurrence in liver surgery. In orthotopic transplantation, the donor liver is exposed to periods of ischemia and when oxygenated blood is reintroduced to the liver, oxidative stress may develop and lead to graft failure. The aim of this project was to investigate whether noninvasive multiphoton and fluorescence lifetime imaging microscopy, without external markers, were useful in detecting early liver damage caused by I/R injury. Localized hepatic ischemia was induced in rats for 1 h followed by 4 h reperfusion. Multiphoton and fluorescence lifetime imaging microscopy was conducted prior to ischemia and up to 4 h of reperfusion and compared to morphological and biochemical assessment of liver damage. Liver function was significantly impaired at 2 and 4 h of reperfusion. Multiphoton microscopy detected liver damage at 1 h of reperfusion, manifested by vacuolated cells and heterogeneous spread of damage over the liver. The damage was mainly localized in the midzonal region of the liver acinus. In addition, fluorescence lifetime imaging showed a decrease in cellular metabolic activity. Multiphoton and fluorescence lifetime imaging microscopy detected evidence of early I/R injury both structurally and functionally. This provides a simple noninvasive technique useful for following progressive liver injury without external markers.
缺血再灌注（I/R）损伤是肝外科的常见并发症。在原位移植中，供体肝脏会经历一段时间的缺血，当含氧血液重新输入肝脏时，氧化应激可能会发生，导致移植物衰竭。本项目旨在研究非侵入性多光子和荧光寿命成像显微镜是否可以在不使用外部标记物的情况下，用于检测由 I/R 损伤引起的早期肝损伤。在大鼠中诱导局部肝缺血 1 小时，然后再灌注 4 小时。在缺血前和再灌注达 4 小时时进行多光子和荧光寿命成像显微镜检查，并与肝损伤的形态和生化评估进行比较。再灌注 2 和 4 小时时，肝功能明显受损。多光子显微镜在再灌注 1 小时时检测到肝损伤，表现为空泡化细胞和损伤在肝脏中不均匀分布。损伤主要局限于肝小叶的中带区。此外，荧光寿命成像显示细胞代谢活性下降。多光子和荧光寿命成像显微镜在结构和功能上均检测到早期 I/R 损伤的证据。这提供了一种简单的非侵入性技术，无需外部标记物即可用于跟踪进行性肝损伤。
