School of Chemistry and Chemical Engineering, University of Jinan, No. 106, Jiwei Road, Jinan 250022, China.
Carbohydr Polym. 2013 Jan 30;92(1):394-9. doi: 10.1016/j.carbpol.2012.09.092. Epub 2012 Oct 6.
A novel flow injection chemiluminescence (FI-CL) sensor for the determination of sulfamethoxazole (SMZ) was established which using chitosan/graphene oxide-molecularly imprinted polymers (CG-MIP) as recognition element. The SMZ-CG-MIP was synthesized in acetone as solvent and chitosan/graphene oxide for support, using acrylamide as functional monomer, ethylene glycol dimethacrylate as cross-linker and 2,2-azobisisobutyronitrile as initiator. The CG-MIP showed satisfactory recognition capacity for the SMZ. Then the synthesized CG-MIP was employed as recognition by packing into a lab-made tube connected in FI-CL analyzer to establish a novel CL sensor respectively. The CL intensity responded linearly to the concentration of SMZ in the range 1.0 × 10(-7)mol/L to 2.3 × 10(-3)mol/L with a detection limit of 2.9 × 10(-8)mol/L (3σ), which is lower. The relative standard deviation for the determination of 4.0 × 10(-4)mol/L of SMZ was 1.92% (n=11). The sensor is reusable and has a great improvement in sensitivity and selectivity for CL analysis which applied to the determination of SMZ in drug samples.
建立了一种用于测定磺胺甲恶唑（SMZ）的新型流动注射化学发光（FI-CL）传感器，该传感器使用壳聚糖/氧化石墨烯-分子印迹聚合物（CG-MIP）作为识别元件。SMZ-CG-MIP 在丙酮作为溶剂和壳聚糖/氧化石墨烯作为载体的条件下合成，以丙烯酰胺为功能单体、乙二醇二甲基丙烯酸酯为交联剂、2,2-偶氮二异丁腈为引发剂。CG-MIP 对 SMZ 表现出令人满意的识别能力。然后，将合成的 CG-MIP 作为识别元件填充到实验室自制的管中，连接到 FI-CL 分析仪中，分别建立了一种新型 CL 传感器。CL 强度与 SMZ 的浓度在 1.0×10(-7)mol/L 至 2.3×10(-3)mol/L 的范围内呈线性响应，检测限为 2.9×10(-8)mol/L（3σ），这更低。测定 4.0×10(-4)mol/L SMZ 的相对标准偏差为 1.92%（n=11）。该传感器可重复使用，在 CL 分析中的灵敏度和选择性有了很大提高，可用于药物样品中 SMZ 的测定。
