Institute of Basic Biological Problems, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.
Enzyme Microb Technol. 2011 Oct 10;49(5):453-8. doi: 10.1016/j.enzmictec.2011.08.007. Epub 2011 Sep 7.
This work describes the first step towards combination of the bioreactor with a starch-degrading microbial consortium and hydrogenase electrode (HE) in one unit for electricity generation. For this purpose, the bioreactor for microbial fermentation was designed with a set of electrodes (pH-sensor, Ag|AgCl reference electrode, Pt-electrode, and HE) inside the bioreactor. Potentials of all electrodes and H(2) accumulation were monitored in the system under the precise pH control. Results obtained with the hydrogen-producing microbial consortium indicated that HE generates the potential equal to the H(2)|2H(+) equilibrium potential. Furthermore, HE was able to catalyze the current generation (200 μA) by consuming H(2) gas produced in the microbial consortium from starch. After 220 h of operation, HE retained at least 81% of the initial activity. Calculations of carbon balance indicated that fermentation products were similar in microbial cells without HE and with HE generating the current due to H(2) consumption.
这项工作描述了将生物反应器与淀粉降解微生物群落和氢化酶电极 (HE) 结合在一个单元中以发电的第一步。为此，设计了用于微生物发酵的生物反应器，其中内部设有一组电极（pH 传感器、Ag|AgCl 参比电极、Pt 电极和 HE）。在精确的 pH 控制下，监测系统中所有电极的电势和 H2 积累情况。产氢微生物群落的结果表明，HE 产生的电势等于 H2|2H+平衡电势。此外，HE 能够通过消耗微生物群落中由淀粉产生的 H2 气体来催化电流的产生（200 μA）。经过 220 小时的运行，HE 保持了至少 81%的初始活性。碳平衡计算表明，由于 H2 消耗而产生电流的 HE 与没有 HE 的微生物细胞中的发酵产物相似。
