Maes Mailis, Giménez José Francisco, D'Alessandro Adriana, De Waard Jacobus H
Laboratorio de Tuberculosis, Instituto de Biomedicina, Universidad Central de Venezuela, Caracas,
J Infect Dev Ctries. 2011 Nov 15;5(11):781-5. doi: 10.3855/jidc.1689.
Guidelines recommend storing tuberculin purified protein derivative (PPD) refrigerated. However, especially in developing countries, maintaining the product refrigerated under field conditions can be difficult, limiting its use. Here we determine the effect of prolonged exposure to high temperatures on the potency of human, bovine and avian tuberculin PPD.
Human, bovine and avian tuberculin PPD were stored for several weeks exposed to temperatures ranging from 37º to 100ºC. The potency was evaluated in vivo, in sensitized or naturally infected animals.
Most test situations didn't affect the biological activity of the tuberculin PPDs and only very long and extreme incubations (several days at 100 °C) compromised the potency.
Tuberculin PPD is very stable and can be stored or transported for long periods without refrigeration.
指南建议将结核菌素纯蛋白衍生物（PPD）冷藏保存。然而，尤其是在发展中国家，在野外条件下维持产品冷藏可能很困难，从而限制了其使用。在此，我们确定长时间暴露于高温对人、牛和禽结核菌素PPD效力的影响。
人、牛和禽结核菌素PPD在37º至100ºC的温度下暴露保存数周。在致敏或自然感染的动物体内评估其效力。
大多数测试情况并未影响结核菌素PPD的生物活性，只有非常长时间和极端的孵育（100°C下数天）才会损害其效力。
结核菌素PPD非常稳定，可以在不冷藏的情况下长期储存或运输。
