Bacteriology Laboratory, Department of P.G. Studies and Research in Biological Science, Rani Durgavati University, Pachpedi, Jabalpur 482001, Madhya Pradesh, India.
Enzyme Microb Technol. 2011 Apr 7;48(4-5):416-26. doi: 10.1016/j.enzmictec.2011.02.001. Epub 2011 Feb 26.
The present study deals with immobilization of purified CA and whole cell of Pseudomonas fragi, Micrococcus lylae, and Micrococcus luteus 2 on different biopolymer matrices. Highest enzyme immobilization was achieved with P. fragi CA (89%) on chitosan-KOH beads, while maximum cell immobilization was achieved with M. lylae (75%) on chitosan-NH(4)OH beads. A maximum increase of 1.08-1.18 fold stability between 35 and 55°C was observed for M. lylae immobilized CA. The storage stability was improved by 2.02 folds after immobilization. FTIR spectra confirmed the adsorption of CA on chitosan-KOH beads following hydrophilic interactions. Calcium carbonate precipitation was achieved using chitosan-KOH immobilized P. fragi CA. More than 2 fold increase in sequestration potential was observed for immobilized system as compared to free enzyme. XRD spectra revealed calcite as the dominant phase in biomimetically produced calcium carbonate.
本研究探讨了将纯化的 CA 和弗氏假单胞菌、粘质沙雷氏菌和微球菌的全细胞固定在不同的生物聚合物基质上。在壳聚糖-KOH 珠上,弗氏假单胞菌 CA 的酶固定化率最高(89%),而在壳聚糖-NH(4)OH 珠上,粘质沙雷氏菌的细胞固定化率最高(75%)。在 35 至 55°C 之间,固定化的 M. lylae CA 的稳定性提高了 1.08-1.18 倍。固定化后,储存稳定性提高了 2.02 倍。傅里叶变换红外光谱证实了 CA 通过亲水相互作用吸附在壳聚糖-KOH 珠上。利用壳聚糖-KOH 固定化的弗氏假单胞菌 CA 实现了碳酸钙的沉淀。与游离酶相比,固定化体系的螯合潜力增加了两倍以上。XRD 图谱显示生物模拟生成的碳酸钙中以方解石为主。