Warren D W, Pasupuleti V, Lu Y, Platler B W, Horton R
Department of Physiology and Biophysics, University of Southern California, School of Medicine, Los Angeles 90033.
J Androl. 1990 Jul-Aug;11(4):353-60.
The actions of two cytokines, tumor necrosis factor (TNF) and interleukin-1 (IL-1), on testosterone production by dispersed adult testis cells and purified Leydig cells in culture were studied. In one set of experiments, testis cells from adult (90-day-old) rats were enzymatically dispersed. In another set of experiments, the dispersed testis cells were placed on a Percoll density gradient and were centrifuged to yield purified (greater than 85%) Leydig cells. Both whole testis cells and purified Leydig cells were cultured in the presence of varying doses of TNF or IL-1 with or without maximally stimulating doses of human chorionic gonadotropin (hCG). Both TNF and IL-1 stimulated basal secretion of testosterone in whole testis cells, as well as purified Leydig cells. Additionally, both TNF and IL-1 augmented maximally hCG stimulated testosterone secretion. Both cytokines stimulated testosterone secretion by dispersed testis cells as early as 4 hours, and the effect continued for up to 72 hours. The cytokines slightly, but significantly, stimulated testosterone production in purified Leydig cells after 24 hours, and continued for up to 72 hours. We have concluded from this data that TNF and IL-1 stimulate the testosterone secretion by adult rat Leydig cells. While this effect might be mediated through the action of the cytokines on testicular macrophages, there might also be a direct effect on the Leydig cell since augmentation of secretion occurred in purified Leydig cells, as well as whole testis cells. Therefore, TNF and IL-1 may serve as local regulators of Leydig cell function.
研究了两种细胞因子，即肿瘤坏死因子（TNF）和白细胞介素-1（IL-1），对成年睾丸分散细胞和培养的纯化莱迪希细胞睾酮分泌的作用。在一组实验中，将成年（90日龄）大鼠的睾丸细胞进行酶分散处理。在另一组实验中，将分散的睾丸细胞置于Percoll密度梯度上并离心，以获得纯化的（大于85%）莱迪希细胞。将完整的睾丸细胞和纯化的莱迪希细胞在不同剂量的TNF或IL-1存在下培养，同时添加或不添加最大刺激剂量的人绒毛膜促性腺激素（hCG）。TNF和IL-1均刺激了完整睾丸细胞以及纯化莱迪希细胞中睾酮的基础分泌。此外，TNF和IL-1均增强了hCG最大刺激下的睾酮分泌。两种细胞因子早在4小时就刺激了分散睾丸细胞的睾酮分泌，且该作用持续长达72小时。细胞因子在24小时后对纯化莱迪希细胞的睾酮分泌有轻微但显著的刺激作用，并持续长达72小时。我们从这些数据中得出结论，TNF和IL-1刺激成年大鼠莱迪希细胞的睾酮分泌。虽然这种作用可能是通过细胞因子对睾丸巨噬细胞的作用介导的，但由于纯化的莱迪希细胞以及完整睾丸细胞中均出现了分泌增加，因此可能对莱迪希细胞也有直接作用。因此，TNF和IL-1可能作为莱迪希细胞功能的局部调节因子。
